| 水稻苹果酸脱氢酶基因的克隆及其在E.coli中的表达 |
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| 引用本文： | 姜瑞华,林长发,申国安,钱旻,王东,杜喜玲,黄骥,杨金水.水稻苹果酸脱氢酶基因的克隆及其在E.coli中的表达[J].复旦学报(自然科学版),2002,41(1):67-69. |
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| 作者姓名： | 姜瑞华 林长发 申国安 钱旻 王东 杜喜玲 黄骥 杨金水 |
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| 作者单位： | 复旦大学生命科学学院,遗传学研究所,上海,200433 |
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| 摘 要： | 以玉米苹果酸脱氢酶的基因为探针，从水稻幼苗cDNA文库筛选得到一条水稻新基因，它与玉米ZHO2序列的一致性高达87％，被命名为RcMDH(rice cytoplasmic malic dehydrogenase gene)，分析发现RcMDH具有完整的读码框，编码332个氨基酸，预计分子质量为36．5ku,导入大肠杆菌中表达，经检测证实该产物具苹果酸脱氢酶酶活性。
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| 关 键 词： | 水稻 细胞质 莱果酸 脱氢酶基因 克隆 表达 |
| 文章编号： | 0427-7104(2002)01-0067-03 |
Cloning of the Rice Cytoplasmic Malic Dehydrogenase Gene (RcMDH) and Its Expression in E. coli |
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| JIANG Rui hua,LIN Chang fa,SHEN Guo an,QIAN Min,WANG Dong,DU Xi ling,HUANG Ji,YANG Jin shui.Cloning of the Rice Cytoplasmic Malic Dehydrogenase Gene (RcMDH) and Its Expression in E. coli[J].Journal of Fudan University(Natural Science),2002,41(1):67-69. |
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| Authors: | JIANG Rui hua LIN Chang fa SHEN Guo an QIAN Min WANG Dong DU Xi ling HUANG Ji YANG Jin shui |
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| Abstract: | Using the maize MDH gene as probe, a novel rice full length cDNA was cloned through screening a rice seedling cDNA library. It showed 87% identity in nucleic acid sequence to ZHO 2(GenBank No.AF007581)of maize, and therefore was named as rice cytoplasmic malic dehydrogenase gene( RcMDH ). This gene encodes 322 amino acids, with putative molecular weight of 36.5 ku. The open reading frame of RcMDH was subcloned into reconstructed pGEX 4T 1 plasmid which expressed in E.coli with IPTG inducing. The MDH enzyme activity of its product was also certified. |
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| Keywords: | rice cytoplasmic malic dehydrogenase gene ( RcMDH ) clone expression |
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