|
|||||
|
|
| 环境污染地区成年男性精子microRNA表达谱的建立 | |
| 引用本文： | 于洋,李燕,梁雨蒙,陈德宇.环境污染地区成年男性精子microRNA表达谱的建立[J].阜阳师范学院学报(自然科学版),2011,28(3):54-61. |
| 作者姓名： | 于洋 李燕 梁雨蒙 陈德宇 |
| 作者单位： | 1. 吉林市第五人民医院,吉林吉林,132011 2. 宁波大学医学院,浙江宁波,315211 3. 阜阳师范学院生命科学学院,安徽阜阳,236041 |
| 基金项目： | 国家自然科学基金(31071317);教育部科学技术研究重点项目(210104);宁波市自然科学基金(2011A610060);宁波大学大学生科技创新(SRIP)重点项目资助 |
| 摘 要： | 通过收集污染区男性精液标本30份和30份对照地区成年男性的精液标本,提取精子RNA,标记后与miRCU-RYTM LNA Array芯片杂交,分析环境污染与对照地区成年男性精子中miRNA的表达情况。结果表明环境污染与对照地区男性精子共182个miRNA存在表达差异,在环境污染地区73个miRNAs显著上调,109个m... |
| 关 键 词： | microRNA 微阵列 精子 环境污染 |
Construction of MicroRNA profiles in adult man sperms of environment pollution site |
|
| YU Yang,LI Yan,LIANG Yu-meng,CHEN De-yu.Construction of MicroRNA profiles in adult man sperms of environment pollution site[J].Journal of Fuyang Teachers College:Natural Science,2011,28(3):54-61. | |
| Authors: | YU Yang LI Yan LIANG Yu-meng CHEN De-yu |
| Institution: | 1.The Fifth Hospital of Jilin City,Jilin Jilin 132011,China; 2.School of Medicine,Ningbo University,Ningbo Zhejiang 315211,China; 3.School of Life Science,Fuyang Teachers College,Fuyang Anhui 236041,China) |
| Abstract: | 30 semen samples from electronic waste polluted environment and normal control environment site.Total RNA were harvested using TRIzol(Invitrogen) and RNeasy mini kit(QIAGEN) according to manufacturer’s instructions.Samples were labelled using the miRCURYTM Hy3TM/Hy5TM Power labelling kit and hybridized on the miRCURYTM LAN Array(v.14.0).The samples were hybridized on a hybridization station.There were 73 significantly up-regulated miRNA and 109 down-regulated miRNA in the expression profile of adult man sperms in electronic waste polluted environment compared with the miRNA expression profile of adult man sperms in normal control environment.Construction of miRNA profiles in adult man sperm of environmental polluted sites can help to analyze the molecular mechanism of the pathogenesis of spermatogenesis by environmental pollution. |
| Keywords: | microRNA microarrays sperm environment pollution |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |