| 甘蓝型油菜BnTR1和ATP6之间相互作用的研究 |
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| 引用本文： | 严碧云,刘志斌,向俊蓓,岳渝飞,杨毅.甘蓝型油菜BnTR1和ATP6之间相互作用的研究[J].四川大学学报(自然科学版),2011,48(2):457-461. |
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| 作者姓名： | 严碧云 刘志斌 向俊蓓 岳渝飞 杨毅 |
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| 作者单位： | 四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都,610064 |
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| 基金项目： | 国家自然科学基金（30971557，30971816） |
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| 摘 要： | 为进一步研究ATP6和BnTR1之间的相互作用及具体作用位点,通过构建含不同长度的BnTR1 cDNA序列的pGADT7融合载体,利用酵母双杂交证明了BnTR1的C端92～202位氨基酸序列与ATP6的氨基酸序列具有相互作用.
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| 关 键 词： | 酵母双杂交 甘蓝型油菜(Brasscia napus L.) ATP6 BnTR1 |
Research on the interaction between BnTR1 and ATP6 of Brassica napus L. |
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| YAN Bi-Yun,LIU Zhi-Bin,XIANG Jun-Bei,YUE Yu-Fei and YANG Yi.Research on the interaction between BnTR1 and ATP6 of Brassica napus L.[J].Journal of Sichuan University (Natural Science Edition),2011,48(2):457-461. |
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| Authors: | YAN Bi-Yun LIU Zhi-Bin XIANG Jun-Bei YUE Yu-Fei and YANG Yi |
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| Institution: | Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University;Key Laboratory of Bio-resources and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University |
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| Abstract: | In order to analyzed the interacting area and point between ATP6 and BnTR1, different length BnTR1 cDNA sequence fusion vector was constructed by pGADT7 plasmid. The results showed that the interacting area with ATP6 amino acid sequence pointed at 92~202 aa of C end in BnTR1 amino acid sequence. |
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| Keywords: | ATP6 BnTR1 |
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