用SEM-EDXA技术测定麦草纤维细胞木素分布

<正>建立了一种用带有能谱仪的扫描电子显微镜(SEM-EDXA) 测定麦草细胞壁木素分布的方法。讨论了溴化、切片厚度和样品座对定量测定的影响。结果表明,溴对木素的化合不但是专一的,而且也是稳定的;利用薄切片背散射,吸收和荧光对定量测定的影响可以忽略,空间分辩力也可得到弥补。因此,SEM-EDXA技术以较高的准确度提供了定量测定木素分布的信息。

LIGNIN DISTRIBUTION IN WHEAT STRAW FIBER AS DETERMINED BY SEM-EDXA TECHNIQUE

A method has been developed for the determination of lignin distribution in wheat straw cell walls by scanning electron microscopy coupled with energy dispersive X-ray analysis (SEM-EDXA) technique. This paper deals with the effects of bromination, section thickness and specimen grid holder on quantitative assay of lignin distribution. Results indicate that the combination of bromine for lignin is not only specific but stable, and the effects of backscattering. absorption and fluoresence on the quantitative analysis may all be ignored and the spatial resolution can be remedied by using thin speeimens. Therefore, the SEM-EDXA technique provides quantitative information of lignin distribution with relatively high accuracy.