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| 一株软骨霉状菌的纯化方法 | |
| 引用本文： | 郭秋菊,Ronald Garcia,钱晓鸣,沈月毛. 一株软骨霉状菌的纯化方法[J]. 厦门大学学报(自然科学版), 2011, 50(1): 106-110 |
| 作者姓名： | 郭秋菊 Ronald Garcia 钱晓鸣 沈月毛 |
| 作者单位： | 1. 厦门大学,生命科学学院,福建省药物工程实验室,福建,厦门,361005 2. 萨尔大学生物医药与工程研究所,德国,萨尔布吕肯,5066041 3. 厦门大学,生命科学学院,福建省药物工程实验室,福建,厦门,361005;厦门大学细胞生物学与肿瘤细胞工程教育部重点实验室,福建,厦门,361005 |
| 基金项目： | 科技部国际科技合作项目(2007DFA30970) |
| 摘 要： | 从武夷山的一枯枝上分离得到了一株稀有软骨霉状菌,在培养过程中发现,该株黏细菌在人工培养基上只有在与一种未知的细菌共培养的状态下才能长出典型的子实体并保持菌种的活力.然而要从共培养的菌落中纯化这株黏细菌,使用常规的黏细菌纯化技术几乎是不可能.在这种情况下对伴生细菌进行16SrDNA菌种鉴定,再根据鉴定为施氏假单胞菌(Ps... |
| 关 键 词： | 软骨霉状菌 16S rDNA菌种鉴定 施氏假单胞菌 黏细菌纯化 |
The Purification of Chondromyces sp. |
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| GUO Qiu-ju,Ronald Garcia,QIAN Xiao-ming,SHEN Yue-mao. The Purification of Chondromyces sp.[J]. Journal of Xiamen University(Natural Science), 2011, 50(1): 106-110 | |
| Authors: | GUO Qiu-ju Ronald Garcia QIAN Xiao-ming SHEN Yue-mao |
| Affiliation: | 1,3(1.School of Life Sciences,Xiamen University,Laboratory of Microbial Pharmaceutical Engineering,Xiamen 361005,China;2.Nstitut für Pharmazeutische Biotechnologie,Universitt des Saarlandes,5066041 Saarbrücken,Germany;3.Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,Xiamen University,Xiamen 361005,China) |
| Abstract: | The Chondromyces sp.was isolated from a piece of rotting wood collected in Wuyi Mountains in August 2008.In the process of purification,we found that Chondromyces sp.could not live and produce fruiting bodies unless cultivated together with an unknown bacteria.Once the two species grew together,the unknown bacteria could be purified easily but not the Chondromyces sp.by traditional methods.Later,the unknown bateria was identified as Pseudomonas stutzeri using 16S rDNA sequense identification.It belongs to a... |
| Keywords: | Chondromyces sp. 16S rDNA sequense identification Pseudomonas stutzeri Myxobacterium purification |
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