海产干货中克罗诺杆菌污染分布调查及生物学特性研究

克罗诺杆菌是一种革兰氏阴性菌,该菌主要寄生于人和动物的肠道,易引发新生儿脑膜炎、菌血症及坏死性小肠结肠炎等一系列高致死率疾病。通过改进的国家标准检测方法开展120种海产干制品中克罗诺杆菌的污染调查,并对分离菌株进行生物学特性研究。结果显示:克罗诺杆菌的检出率为17.83%,进一步研究23株分离菌株的菌膜形成能力与鞭毛运动能力,11号菌株的菌膜形成能力最强并且有较强的运动性。研究表明:海产干货制品中克罗诺杆菌污染具有较高的污染率,菌株表现出不同程度的生物膜形成能力和运动性,说明该菌污染干燥海产品干货可能与生物膜形成有关,为预防与控制海产干货制品中克罗诺杆菌的污染提供了基础数据。

Distribution and Biological Characterization of Cronobacter spp. in Dry Aquatic Food Samples

Cronobacter spp. is a group of foodborne pathogens causing serious infections including neonatal meningitis, necrotizing enterocolitis and bacteremia with high fatality rate. In this study, 120 dry seafood samples were collected to study the contamination investigation of Cronobacter spp. and further study on biological characterization was performed. The detection rate of Cronobacter spp. was 17.83%. Then, the relationship between biofilm forming abilities and motility of 23 isolates were explored. Meanwhile, the No.11 strain had the strongest biofilm-forming ability and relatively stronger motility than most of the isolates. Results showed that the contamination rate of Cronobacter spp. in dry aquatic food was high and different strains had different biofilm forming abilities and motilities, which provided important basic data for the prevention and control of Cronobacter spp. in food production.