HPLC法直接拆分dracocephinsA对映异构体

建立了一种直链淀粉键合手性固定相拆分 ｄｒａｃｏｃｅｐｈｉｎｓＡ对映异构体的方法。使用 ＣｈｉｒａｌｐａｋＩＡ柱（４６ｍｍ×２５０ｍｍ，５μｍ），流动相为正己烷 －乙酸乙酯（１０∶９０），流速１０ｍＬ·ｍｉｎ^－１，检测波长 ２９０ｎｍ，柱温为室温。所建立的方法能有效实现 ｄｒａｃｏｃｅｐｈｉｎｓＡ两对对映异构体的同时拆分，通过 ＣＤ检测器检测结合与单一构型异构体的比对，可以确定４种异构体出峰顺序。直链淀粉键合手性固定相可以用于 ｄｒａｃｏｃｅｐｈｉｎｓＡ对映异构体的手性分离。

HPLC separation of enantiomers of dracocephins A

To establish a direct enantioseparation method by HPLC on immobilized amylose-based chiral stationary phase for the chiral separation of the enantiomers of dracocephins A. The separation was carried out using a Chiralpak IA column （4. 6 mm ×250 mm, 5μm） in combination with n-hexane-ethyl acetate （ 10： 90） as mobile phase. The flow rate was 1. 0 mL · min^-1 and detection wavelength was 290nm, whilst the column temperature was room temperature. The established chromatographic method was effective for the simultaneous separation of the two pairs of enantiomers of dracocephins A. The elution order was determined by coupling HPLC-CD detection and comparison with each single en- antiomer. Immobilized amylose-based chiral stationary phase is suitable for the enantioseparation of dracocephins A.