Quantitation of proinsulin mRNA sequences in hamster insulinoma cells in culture by molecular hybridization |
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Authors: | H Yamamoto K Nose N Itoh H Okamoto |
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Institution: | (1) Department of Biochemistry, Toyama Medical and Pharmaceutical University School of Medicine, Toyama, 930-01 Toyama, (Japan) |
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Abstract: | Summary The content of proinsulin mRNA sequences was measured in a cultured cell line established from a transplantable hamster islet cell tumor, by hybridization with proinsulin cDNA. The cells cultured in vitro were found to contain a significant amount of proinsulin mRNA sequences, compared with non-insulin-producing hamster tissues, and seem to be a useful system for the study of insulin gene expression.This work was supported in part by Grants-in-Aid Scientific Research (248129, 387065, 337013, 378052) and a Grant-in-Aid for Cancer Research (301538) from the Ministry of Education, Science and Culture, Japan. |
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