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In situ hybridization of rDNA on chromosomes of the honeybee,Apis mellifera L.
Authors:M. Beye  R. F. A. Moritz
Affiliation:(1) Institut für Biologie, FB 14, Technische Universität Berlin, Franklinstr. 28/29, D-1000 Berlin 10, (Federal Republic of Germany)
Abstract:DNA probes containing the repeated rDNA region ofDrosophila melanogaster (coding for e.g. 28S and 18S rRNA) hybridized in situ to distinct regions of two heterologous mitotic chromosomes of the honeybee, identifying the nucleolus organizing regions (NORs). The method allows a rapid establishment of a physical map ofApis mellifera using other DNA probes ofDrosophila. This is the first report on well-defined chromosomal markers in the honeybee.
Keywords:Apis mellifera  fluorescence in situ hybridization  rDNA  chromosome
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