顺序注射-阀上实验室-化学发光法测定生物样品中的ROS

活性氧ROS是生理过程必要的代谢产物，适量的ROS起到信号转导作用，一旦过量会对细胞、蛋白质和DN A 造成损伤．在顺序注射-阀上实验室-化学发光系统内，优化并评价了鲁米诺-Co2＋-ROS和鲁米诺-HRP-ROS 2种化学发光体系对ROS的检测条件和分析性能，并将其应用于肝细胞和植物叶片ROS的测定．结果表明，顺序注射-阀上实验室-ROS-化学发光检测法具有自动化程度高、分析速度快、重现性好和试剂试样用量少的显著特点，其中鲁米诺-Co2＋-ROS 体系灵敏度高，相对发光强度与ROS浓度在0．5～1000 nmol · L -1范围内的回归方程为Y ＝49X＋1700（R2＝0．9924），检出限为0．27 nmol · L -1，对细胞和植物组织中 ROS 的回收率在95％～104％之间，结果令人满意．

Sequential injection lab-on-valve integrating chemiluminescent method for the determination of ROS in biologic matters

Reactive oxygen species （ROS） are physiological metabolites ,appropriate amount ROS play an essential role in signal transduction ,but excess ROS can damage cellular components such as pro-teins ,lipids ,and DNA .In this work ,employing sequential injection lab-on-valve chemiluminescent technique（SIA-LOV-CL） ,we explored and optimized the factors influencing Luminol-Co2＋-ROS and Luminol-HRP-ROS chemiluminescent system ,respectively .We also applied the proposed method for the determination of ROS in Chang liver cells and plant tissue .The results showed that SIA-LOV-CL system for ROS determination not only possessed high sensitivity and stability ,but also minimized sample and reagent consumption .The relative CL intensity for Luminol-Co2＋-ROS system was linear with ROS concentration in the range of 0 .5~1000 nmol · L -1 ,and a 0 .27 nmol · L -1 detection limit was achieved .The recoveries for the determination of ROS in Chang liver cells and plant tissue were in the range of 95% ~104% .