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| 几种水稻籼型恢复系和不育系离体培养和遗传转化的研究 | |
| 引用本文： | 易自力,严钦泉,邓启云,刘选明,周朴华,李祥,储成才.几种水稻籼型恢复系和不育系离体培养和遗传转化的研究[J].湖南大学学报(自然科学版),2002,29(1). |
| 作者姓名： | 易自力 严钦泉 邓启云 刘选明 周朴华 李祥 储成才 |
| 作者单位： | 1. 湖南农业大学生物技术系,湖南长沙,410128 2. 国家杂交水稻工程技术研究中心,湖南长沙,410128 3. 湖南大学生物技术研究院,湖南长沙,410082 4. 中国科学院遗传所,北京,100101 |
| 摘 要： | 对二系杂交水稻亲本籼型恢复系 2 88,198,112 5和籼型不育系株 1- s进行了离体培养和遗传转化的研究 ,建立了这些籼稻新品系的胚性愈伤组织高频诱导与再生系统及其基因枪转化系统 .它们的胚性愈伤组织诱导率分别可达 87.4 % ,90 .6% ,78.9%和 88.0 % ;其分化率分别可达 60 .0 % ,4 5 .8% ,4 6.9%和 60 .6% ;其基因枪转化率分别可达 16.69% ,2 .65 % ,2 .0 4 %和 10 .78% ,并获得了一批可育的水稻转基因植株 |
| 关 键 词： | 籼稻 恢复系 不育系 离体培养 基因枪转化 |
Studies on in Vitro Culture and Genetic Transformation of Several Indica Restorer Lines and Sterile Line |
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| YI Zi li,YAN Qin quan,DENG Qi yun,LIU Xuan ming,ZHOU Pu hu,LI Xiang,CHU Cheng cai.Studies on in Vitro Culture and Genetic Transformation of Several Indica Restorer Lines and Sterile Line[J].Journal of Hunan University(Naturnal Science),2002,29(1). | |
| Authors: | YI Zi li YAN Qin quan DENG Qi yun LIU Xuan ming ZHOU Pu hu LI Xiang CHU Cheng cai |
| Institution: | YI Zi li 1,YAN Qin quan 1,DENG Qi yun 2,LIU Xuan ming 3,ZHOU Pu hua 1,LI Xiang 1,CHU Cheng cai 4 |
| Abstract: | The new Indica restorer line 288,198,1125 and the male sterile line zhu1 s are the very important parents of two line hybrid rice.The in vitro culture and the genetic transformation of them were investigated. The high frequency system of regeneration and particle bombardment transformation for embryogenic callus of them was established. Their highest frequency of initiation for embryogenic callus is 87.4%,90.6%,78.0% and 88.0% respectively; Their highest frequency of regeneration for embryogenic callus is 60.0%,45.8%,46.9% and 60.6% respectively; their frequency of particle bombardment transformation is 16.69%,2.65%,2.04% and 10.78% respectively. Some of fertile transgenic rice plants are obtained. |
| Keywords: | indica rice restorer line sterile line in vitro culture biolistic bombardment |
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