| Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment |
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| 引用本文： | 易自力,Wang Li,Cao Shouyun,Yuan Longping,Guan Chunyun,Zhau Puhan,Tian Wenzhang,Chu Chengcai. Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment[J]. 高技术通讯(英文版), 2003, 9(1): 6-10 |
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| 作者姓名： | 易自力 Wang Li Cao Shouyun Yuan Longping Guan Chunyun Zhau Puhan Tian Wenzhang Chu Chengcai |
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| 作者单位： | [1]HunanAgriculturalUniversity,Changsha410128,P.R.China [2]InstituteofGenetics,ChineseAcademyofSciences,Beijing100101,P.R.China |
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| 基金项目： | the Chinese 863 High-Technology Program(NO.863-101-01-01-01)and Chinese Post-Doctoral Science Foundation(2001-14) |
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| 摘 要： | In order to improve the frequency of indica rice transformation by biolistic bombardment,suitable culture conditions for embryonic calli,an optimal selection scheme for resistant calli and seedling,and optimum bombardment parameters a investigated by using 14 commercially important indica rice cultivars.The main results show that the CC medium with 36g/L mannitol is a scheme subculture medium in which the browning of indica rice calli can be mitigated significantly;The concentration of 30-40mg/L Hyg or 150-200mg/L G418 or 10-20mg/L Basta is suitable for selection of resistant calli;The transformation parameters of 100μg gold powder absorbing 0.2μg DNA per shot and 900 psi helium pressure and 6 cm bombardment distance and bombarded twice for each plate give the best result;Keeping the target calli on osmotic medium containing 60g/L mannitol from 12-24h before bombardment to 24-48h after it can increase the efficiencies of transformation.Furthermore,some transgenic indica rice plants are obtained using this optimized transformation system.
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| 关 键 词： | 水稻 遗传育种 胚胎培养 愈伤组织 培养基 粒子轰击 转化频率 |
Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment |
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| Wang Li,Cao Shouyun,Yuan Longping,Guan Chunyun,Zhau Puhan,Tian Wenzhang,Chu Chengcai. Studies of Improving the Frequency of Indica Rice Transformation by Biolistic Bombardment[J]. High Technology Letters, 2003, 9(1): 6-10 |
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| Authors: | Wang Li Cao Shouyun Yuan Longping Guan Chunyun Zhau Puhan Tian Wenzhang Chu Chengcai |
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| Abstract: | In order to improve the frequency of indica rice transformation by biolistic bombardment, suitable culture conditions for embryonic calli,an optimal selection scheme for resistant calli and seedlings, and optimum bombardment parameters a investigated by using 14 commercially important indica rice cultivars. The main results show that the CC medium with 36g/L mannitol is a scheme subculture medium in which the browning of indica rice calli can be mitigated significantly; The concentration of 30～40mg/L Hyg or 150～200mg/L G418 or 10～20 mg/L Basta is suitable for selection of resistant calli; The transformation parameters of 100μg gold powder absorbing 0.2μg DNA per shot and 900 psi helium pressure and 6 cm bombardment distance and bombarded twice for each plate give the best result; Keeping the target calli on osmotic medium containing 60g/L mannitol from 12 ～24h before bombardment to 24～48h after it can increase the efficiencies of transformation . Furthermore, some transgenic indica rice plants are obtained using this optimized transformation system. |
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| Keywords: | indica rice biolistic bombardment transformation callus |
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