基于微生物诱导碳酸钙的岩体结构面修复试验

针对混合花岗岩开展了巴氏芽孢杆菌诱导碳酸钙沉积的结构面修复试验研究.在实验室内对巴氏芽孢杆菌活化培养,利用电导率变化量表征巴氏芽孢杆菌脲酶活性,考察其生长规律与理化特性.借助XRD,EDS及SEM等手段对结构面处巴氏芽孢杆菌诱导产物进行分析.研究表明,选用22~28h培养后的菌液,巴氏芽孢杆菌脲酶活性最高;30℃,pH为8的理化环境最适合巴氏芽孢杆菌生长繁殖;巴氏芽孢杆菌在结构面处的沉积产物为5~10μm的立方体状碳酸钙晶体,以螺旋位错结晶方式生长,最终将结构面胶结在一起,达到结构面修复的目的.

Healing Experiment for Rock Mass Structural Plane Based on Bacterially Induced Calcium Carbonate

Sporosarcina pasteurii-induced calcium carbonate precipitation was used to heal structural planes of migmatitic granite. Conductivity variations was applied to characterize the urease activity for bacteria liquid to investigate the growth law and physicochemical properties of Sporosarcina pasteurii after curing in activated culture. Additionally, microscopic methods including XRD, EDS and SEM were conducted to analyze the induced products of Sporosarcina pasteurii on structural planes. The results show that, the urease activity for bacteria liquid of Sporosarcina pasteurii is the highest after curing 22 ~ 28 hours. The optimum physicochemical environment for Sporosarcina pasteurii growth is 30℃ at pH 8. The sediment product produced by Sporosarcina pasteurii on structural planes is cubic crystals with a side length of 5~10μm, and they grow in a way of screw dislocation to backfill and then repair structures planes.