| 新型肾综合征出血热EIA诊断试剂盒的研制 |
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| 引用本文： | 李铮,杨水云.新型肾综合征出血热EIA诊断试剂盒的研制[J].陕西师范大学学报,2001,29(2):58-62. |
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| 作者姓名： | 李铮 杨水云 |
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| 作者单位： | [1]陕西师范大学化学与材料科学学院，陕西西安710062 [2]西安交通大学生物科学与工程系，陕西西安710049 |
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| 基金项目： | 陕西省自然科学基金资助项目(691049) |
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| 摘 要： | 用多肽合仪合成汉坦病毒核衣壳蛋白（NP）(aa17－66）50个氨基酸残基的核心序列，以此为抗原，建立特羿性检测抗汉坦病毒IgG和IgM的酶联免疫吸附剂测定法（ELISA），通过实验发现这一序列具有较强的抗原性，研制出了以合成肽为抗原的新型牧场异性检测抗汉坦病毒IgG和IgM的诊断试剂盒，通过对肾综合征出血热抗体阳性血清的检测，效果良好。
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| 关 键 词： | 肾综合征出血热 抗原表位 多肽合成 诊断试剂盒 |
| 文章编号： | 1001-3857(2001)02-0058-05 |
| 修稿时间： | 2000年6月17日 |
Development of a new type HFRS EIA diagnostic kit |
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| LI Zheng ,YAN Shui-yun.Development of a new type HFRS EIA diagnostic kit[J].Journal of Shaanxi Normal University: Nat Sci Ed,2001,29(2):58-62. |
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| Authors: | LI Zheng YAN Shui-yun |
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| Institution: | LI Zheng 1,YAN Shui-yun 2 |
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| Abstract: | Hantaviruses (HV), belonging to the family Bunyaviridae, cause hemorrhagic fever with syndrome (HFRS). HFRS is characterized by fever, renal dysfunction and bleeding complications. Small mammals, the mainly rodents, are the natural reservoirs. In order to establish a new specific serological diagnostic kit to test HFRS. The aa17-66 of NP epitope has been synthesized by solid phase peptide synthesis .Then the synthetic peptide is applied as an antigen to test the specific antibody (IgG,IgM) by EIA. The synthetic peptide shows stronger high antigenic. The anti-HV IgM and IgG are detected specially to develop a specific EIA kit for the diagnosis of HFRS. |
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| Keywords: | HFRS antigenic epitope peptide synthesis HFRS EIA diagnostic kit |
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