| 怀地黄85-5品种内遗传多样性的RAPD和ISSR分析 |
| |
| 引用本文： | 周延清,牛敬媛,田苗苗,李振勇,崔天星,邵大晓,李丙亮,冯艳铭,李智涛,李争,王天亮,范喜梅,李敏,申远.怀地黄85-5品种内遗传多样性的RAPD和ISSR分析[J].河南师范大学学报(自然科学版),2006,34(3):136-139. |
| |
| 作者姓名： | 周延清 牛敬媛 田苗苗 李振勇 崔天星 邵大晓 李丙亮 冯艳铭 李智涛 李争 王天亮 范喜梅 李敏 申远 |
| |
| 作者单位： | 河南师范大学,生命科学院,河南,新乡,453007;华美生物工程公司,河南,洛阳,471003;温县农业科学研究所,河南,温县,454881;焦作师范高等专科学校,河南,焦作,454000 |
| |
| 摘 要： | 采用CTAB法提取了怀地黄85-5品种16个单株的基因组DNA,使用紫外分析和琼脂糖凝胶电泳检测其纯度和大小,利用RAPD及ISSR分析其品种内遗传多样性.从24个RAPD引物和43个ISSR引物中分别筛选出具有稳定多态性条带的RAPD引物3个和ISSR引物2个,分别对16个单株基因组DNA进行PCR扩增.3个RAPD引物共扩增出7条带,其中多态性条带为4个,多态性比率为57.14%.2个ISSR引物共扩增出17条带,其中多态性条带为11个,多态性比率为64.7%.结果表明,怀地黄85-5品种内存在遗传差异,但遗传多样性比地黄品种间的低.
|
| 关 键 词： | 怀地黄 RAPD ISSR 遗传多样性 |
| 文章编号： | 1000-2367(2006)03-0136-04 |
| 收稿时间： | 2005-07-27 |
| 修稿时间： | 2005年7月27日 |
Genetic Diversity within Rehmannia Glutinosa Libosh. f .Hueichingensis (Chao et Schih) Hsiao Cultivar 85-5 Detected by RAPD and ISSR Markers |
| |
| ZHOU Yan-qing , NIU Jing-yuan , TIAN Miao-miao , LI Zhen-yong , CUI Tian-xing ,SHAO Da-xiao , LI Bing-liang , FENG Yan-ming , LI Zheng, FAN Xi-mei ,LI Min ,SHEN Yuan.Genetic Diversity within Rehmannia Glutinosa Libosh. f .Hueichingensis (Chao et Schih) Hsiao Cultivar 85-5 Detected by RAPD and ISSR Markers[J].Journal of Henan Normal University(Natural Science),2006,34(3):136-139. |
| |
| Authors: | ZHOU Yan-qing NIU Jing-yuan TIAN Miao-miao LI Zhen-yong CUI Tian-xing SHAO Da-xiao LI Bing-liang FENG Yan-ming LI Zheng FAN Xi-mei LI Min SHEN Yuan |
| |
| Institution: | 1. College of Life Sciences, Henan Normal University, Xinxiang 453007, China; 2. Sino-American Biotechnology Company, Luoyang 471003, China; 3. Jiaozuo Teachers College, Jiaozuo 454000, China |
| |
| Abstract: | The genomic DNAs were extracted by CTAB method from the young leaves of 16 individuals of Rehmannia Glutinosa Libosh.f.Hueichingensis(Chao et Schih) Hsiao cultivar 85-5,whose purities and sizes were tested by UV method and agrose gel electrophoresis,respectively.Genetic diversity within cultivar 85-5 was assessed by RAPD and ISSR Markers.Three RAPD primers and two ISSR primers were selected from twenty-four RAPD ones and from forty-three ISSR ones to generate DNA fingerprints of these 16 individuals.Seven fragments were amplified with the three RAPD primers,four of which were polymorphic.Polymorphic percentage was 57.14%.Seventeen fragments were amplified with the two ISSR primers,eleven of which were polymorphic.Polymorphic percentage was 64.7%.The results showed that genetic difference occurred within cultivar 85-5,but genetic diversity was lower than that among different Rehmannia Glutinosa Libosh cultivars. |
| |
| Keywords: | Rehmannia Glutinosa Libosh f Hueichingensis (Chao et Schih) Hsiao RAPD ISSR genetic diversity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
