| HPLC-PDA法对中成药养心生脉颗粒中丹参素含量的测定 |
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| 引用本文： | 刘芃岩,王洪宇,王英锋,李恬. HPLC-PDA法对中成药养心生脉颗粒中丹参素含量的测定[J]. 河北大学学报(自然科学版), 2006, 26(3): 266-269 |
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| 作者姓名： | 刘芃岩 王洪宇 王英锋 李恬 |
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| 作者单位： | 河北大学,化学与环境科学学院,河北,保定,071002;首都师范大学,化学系,北京,100037 |
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| 摘 要： | 建立了用HPLC_PDA法测定养心生脉颗粒中丹参素含量的方法.采用Symmertry ShieldTMRP18(150 mm×4.6 mm,5μm)色谱柱;以V(甲醇)∶V(水)∶V(冰醋酸)=5∶94.5∶0.5为流动相;流速为1.0 mL/min;检测波长280 nm.丹参素在9.5~152 mg/L内,线性关系良好(R>0.999 9);加标回收率平均为98.9%,RSD为0.97%.为丹参类颗粒的质量综合评价和质量控制提供了简单、准确、可行的分析方法.
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| 关 键 词： | 丹参素 养心生脉颗粒 HPLC-PDA |
| 文章编号： | 1000-1565(2006)03-0266-04 |
| 修稿时间： | 2005-05-23 |
Determination of Salvianolic Acid in the Chinese Patent Medicine Yangxinshengmai Granule by HPLC-PDA |
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| LIU Peng-yan,WANG Hong-yu,WANG Ying-feng,LI Tian. Determination of Salvianolic Acid in the Chinese Patent Medicine Yangxinshengmai Granule by HPLC-PDA[J]. Journal of Hebei University (Natural Science Edition), 2006, 26(3): 266-269 |
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| Authors: | LIU Peng-yan WANG Hong-yu WANG Ying-feng LI Tian |
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| Abstract: | An HPLC method for the determination of salvianolic acid in yangxinshengmai granule was established.Separation of salvianolic acid was carried out on Symmertry Shield~(TM) RP-(18) column,with methanol-water-acetic acid(5∶94.5∶0.5) as mobile phase,and the flow rate was 1.0 mL/min;Determination wavelength was set at 280 nm.Linear relationship was obtained in the range of 9.5～152 mg/L(R>0.999 9).The average recovery was 98.9% and RSD(n=5) was 0.97%.This study provided a simple,accurate and feasible method for the determination of salvianolic acid,and a usable integrated quality evaluation as well as a quality control for yangxinshengmai granule. |
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| Keywords: | salvianolic acid yangxinshengmai granule HPLC-PDA |
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