| 水解酸化-好氧处理含油污水中微生物群落变化研究 |
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| 引用本文： | 包木太,闫广彬,陈庆国,社春安,郭省学,李希明.水解酸化-好氧处理含油污水中微生物群落变化研究[J].中国石油大学学报(自然科学版),2011,35(4). |
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| 作者姓名： | 包木太 闫广彬 陈庆国 社春安 郭省学 李希明 |
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| 作者单位： | 1. 中国海洋大学海洋化学理论与工程技术教育部重点实验室,山东青岛,266100
2. 中国石化胜利油田分公司采油工艺研究院,山东东营,257000 |
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| 基金项目： | 青岛市应用基础研究计划项目，“十一五”国家科技支撑计划项目 |
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| 摘 要： | 为揭示水解酸化-好氧处理系统处理油田采出水过程中生物群落的变化规律,考察对不同水力停留时间(tHAT)下好氧池的生物相,提取水解酸化池和好氧池中菌体的脱氧核糖核酸(DNA),以细菌通用引物对DNA V3高变异区聚合酶链式反应(PCR)扩增后进行变性梯废凝胶电泳(DGGE)分析.结果表明:当水解酸化池和好氧池的水力停留时间分别大于12和16 h时,镜检有高级原生动物,出水CODCr去除率大于58.73%,含油去除率大于98.61%；水解酸化-好氧处理工艺在不同tHRT条件下运行后,在形成的细菌群落中,既有相同的优势群落,也有各自特有的优势群落；随着tHRT的延长,水解酸化池中优势群落的种类和数量增加,当tHRT＞12 h时,优势群落变化较小,形成稳定的细菌群落,出水水质趋于稳定；随着tHRT的延长,好氧处理池中优势群落的种类和数量减少,当tHRT＞16 h时,优势群落变化较小,形成稳定的生态系统,出水水质趋于最佳.
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| 关 键 词： | 微生物群落 水解酸化 好氧处理 含油污水 变性梯度凝胶电泳 |
Study on change of microbial community structure in hydrotic acidification-aerobic biological treatment oily wastewater |
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| BAO Mu-tai,YAN Guang-bin,CHEN Qing-guo,DU Chun-an,GUO Sheng-xue,LI Xi-ming.Study on change of microbial community structure in hydrotic acidification-aerobic biological treatment oily wastewater[J].Journal of China University of Petroleum,2011,35(4). |
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| Authors: | BAO Mu-tai YAN Guang-bin CHEN Qing-guo DU Chun-an GUO Sheng-xue LI Xi-ming |
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| Institution: | BAO Mu-tai1,YAN Guang-bin1,CHEN Qing-guo1,DU Chun-an2,GUO Sheng-xue2,LI Xi-ming2 (1.Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education,Ocean University of China,Qingdao 266100,China,2.Research Institute of Oil Production Technology,Shengli Oilfield,SINOPEC,Dongying 257000,China) |
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| Abstract: | In order to reveal the biological community changes of hydrolytic acidification-aerobic system in oilfield produced water treatment,the biological phase of aerobic tank was observed,and the genomic DNA of microbial communities was extracted.The V3 region in DNA was amplified by the universal primers,and the bacterial community structure of hydrolytic acidification-aerobic system was studied by polymerase chain reaction(PCR) amplification and denaturing gradient gel electrophoresis(DGGE).The results show tha... |
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| Keywords: | microbial community hydrolytic acidification aerobic biological treatment oily wastewater denaturing gradient gel electrophoresis(DGGE) |
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