| 太白红杉总DNA的提取及鉴定 |
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| 引用本文： | 阎桂琴 李珊 等. 太白红杉总DNA的提取及鉴定[J]. 西北大学学报(自然科学版), 2001, 31(1): 53-56 |
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| 作者姓名： | 阎桂琴 李珊 等 |
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| 作者单位： | 西北大学生命科学学院，陕西西安710069 |
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| 摘 要： | 用抗氧剂和螯合剂组合的方法，成功地从太白红杉的叶组织中提取和纯化了总DNA，并对其产率、质量和纯度作了鉴定。用此方法能有效地去除酚类化合物、多糖、萜类化合物和未知化合物的干扰，为太白红杉的分子生物学研究提供了一套迅速、经济和可靠的技术方案。
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| 关 键 词： | 太白红杉 总DNA 抗氧剂 螯合剂 组合提取 RAPD 随机扩增多态性DNA |
| 文章编号： | 1000-274X(2001)01-0053-04 |
| 修稿时间： | 2000-06-02 |
Isolation andcharacterization of total DNA from Larix chinensis |
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| YAN Gui qin,LI Shan,WANG Yi ling,WANG Xu mei,ZHAO Gui fang. Isolation andcharacterization of total DNA from Larix chinensis[J]. Journal of Northwest University(Natural Science Edition), 2001, 31(1): 53-56 |
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| Authors: | YAN Gui qin LI Shan WANG Yi ling WANG Xu mei ZHAO Gui fang |
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| Abstract: | An extraction medium with the combination of antioxidants and chelants, which can remove phenolic compound, polysaccharide and terpenoid, was successfully used to obtain total DNA from the leaf tissues of Larix chinensis beissn. The DNA yields, quality and purity were characterized. These isolated DNA could be used directly for PCR reaction, RFLP and RAPD analysis. A rapid, inexpensive and reliable procedure has been developed for further study of genetic diversity of this species. |
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| Keywords: | Larix Chinensis beissn total DNA extraction an extraction medium with the combination of antioxidants and chelants RAPD(Rahdorn Amplified Polymorphism DNA) |
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