刀额新对虾和日本囊对虾细胞核DNA含量的测定与比较

以刀额新对虾(Metapenaeus ensis)、日本囊对虾(Marsupenaeus japonicus)的血淋巴为材料,以小鸡血细胞核DNA含量(2.50 pg/2c)为参照样,使用Partec PA-I型倍性分析仪并结合外标定法,分别测定了刀额新对虾与日本囊对虾细胞的DNA含量.结果表明,这两种虾的细胞DNA含量分别为小鸡血细胞的1.77倍和1.95倍,换算成绝对含量分别为4.340 pg/2c和4.878 pg/2c.本文讨论了刀额新对虾与日本囊对虾以形态学为特征的分类系统与细胞DNA含量之间的关系,并推测在系统演化上日本囊对虾应比刀额新对虾更为高等.

Measurement and Comparison of Celluar DNA Contents Between Metapenaeus ensis and Marsupenaeus japonicus (Decopoda: Crustacean)

Using the absolute value of DNA content of chicken erythrocyte(2.50 pg/2c) as a comparative standard,DNA content of Metapenaeus ensis and Marsupenaeus japonicus were determined by Ploidy Analyser(Partec PA-I,Partec Germany).The absolute DNA contents of the two species were 4.340 pg/2c and 4.878 pg/2c,respectively.The comparative result revealed that the difference between the two species was not apparent in DNA content,which showed that they were quite close to each other in phylogeny based on morphology.Moreover,because the DNA content of Marsupenaeus japonicus was somewhat higher than that of Metapenaeus ensis,we deduced that the former species was more advanced than the latter in evolutionary relation.This study not only enhanced the function of Ploidy Analyser,but also provided theoretical basis for other studies such as inheritance and breeding research field.