| 肺炎克雷伯氏菌3-羟基丙酸合成关键酶基因过表达与NAD+ |
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| 引用本文： | 曹 宁 李 莎 葛喜珍 田平芳. 肺炎克雷伯氏菌3-羟基丙酸合成关键酶基因过表达与NAD+[J]. 北京化工大学学报(自然科学版), 2014, 41(1): 74-77 |
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| 作者姓名： | 曹 宁 李 莎 葛喜珍 田平芳 |
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| 作者单位： | 1北京化工大学 生命科学与技术学院， 北京100029; 2北京联合大学 生物化学工程学院， 北京100023 |
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| 摘 要： | 在肺炎克雷伯氏菌（Klebsiella pneumoniae）中超表达内源甘油脱水酶基因 dhaB 和醛脱氢酶基因 puuC，同时引入酿酒酵母（Saccharomyces cerevisiae）的3-磷酸甘油脱氢酶基因 GPD1，得到耦合3-羟基丙酸（3-HP）合成与NAD+再生的重组菌 K.p (pET-pk-dhaB-puuC-GPD1)。微氧摇瓶发酵结果显示在15h 时3-HP产量达到1.45 g/L，是携带空载体菌的2.39倍。分析是遗传扰动影响了底物甘油的代谢流量分配，从而提高了3-羟基丙酸的产量。
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| 收稿时间： | 2013-04-24 |
Coupling NAD+ regeneration with overexpression of key enzymes for 3-hydroxypropionic acid biosynthesis in Klebsiella pneumoniae |
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| CAO Ning,LI Sha,GE XiZhen,TIAN PingFang. Coupling NAD+ regeneration with overexpression of key enzymes for 3-hydroxypropionic acid biosynthesis in Klebsiella pneumoniae[J]. Journal of Beijing University of Chemical Technology, 2014, 41(1): 74-77 |
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| Authors: | CAO Ning LI Sha GE XiZhen TIAN PingFang |
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| Affiliation: | 1College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029; 2College of Biochemical Engineering, Beijing Union University, Beijing 100023, China |
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| Abstract: | The glycerol dehydratase coding gene dhaB and the aldehyde dehydrogenase coding gene puuC from Klebsiella pneumoniae, and the 3-phosphoric acid glycerol dehydrogenase coding gene GPD1 from Saccharomyces cerevisiae, have been cloned by the polymerase chain reaction (PCR). Subsequently the recombinant K. pneumoniae K.p( pET-pk-dhaB-puuC-GPD1 ) was acquired. Under micro-oxygen fermentation conditions, it showed peak production of 3-hydroxypropionic acid (1.45 g/L) at 15 h, which is 2.39 times that obtained with the strain harboring the blank plasmid pET-pk. These results suggest that overexpressing dhaB, puuCand GPD1 facilitates 3-HP biosynthesis via reallocation of the metabolic flux in K. |
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