异种表面角膜镜片术和异种穿透性角膜移植术后血清特异性抗体的变化

采用敏感的酶联免疫吸附试验，对４例异种表面角膜镜片术和３例异种穿透性角膜移植术（鹅给猴）术后血清特异性抗体水平的变化进行了动态的观察。发现表面镜片术组于木后第１周出现特异性抗体，术后第３周达高峰，第４周明显下降，穿透性角膜移植术组也于木后第１用出现特异性抗体，第６周达高峰，术后第８周开始下降。临床观察发现４例表面角膜镜片术的木眼于术后１８～２５天出现角膜新生血管，并侵入植片的周边部，拆线后３例术８眼角膜新生血管消退，植片透明，１例因层间上皮植入角膜新生血管未消退，植片轻度水肿混浊。３例穿透角膜移植术的术眼于术后１４～１７天出现角膜新生血管，术后６周植片完全混浊。以上结果表明不仅新鲜角膜组织能诱发受主的体液免疫反应，经过冷冻切削的角膜组织也能诱发机体的体液免疫反应。

CHANGES OF SERUM SPECIFIC ANTIBODIES FOLOWING HETEROEPIKERATOPHAKIA AND PENETRATING HETEROKERATOPLASTY

Nonhuman primate models were established for heteroepikeratophakia and penetriatingheterokeratoplasty by using goose corneal tissue.Serum anti-goose corneal antibodies.weredynamically determined by ELISA,In epikeratophakias,the antibodies appeared in the firstweek after surgery and reached the peak in the third week.The level of serum antibodies,de-creased significantly in the fourth week.The corneal new vessels showed in the peripherial areasof epikeratophakia grafts in the third and fourth week.In penetrating heterokeratoplasties,se-rum antibodies appeared in the first week,reached the peak in the sixth week,and began todecrease,in the eighth week after transplantation.The penetrating grafts were clear forabout a two-week period of surgery,then rapidly rejected,which was characterized bycorneal neovascularizition,aqueous flare,and graft oedema.It was suggested that not only freshpenetrating heterografts but frozen heteroepikeratophakia grafts could elicit the humoral immuni-ty of the hosts.