树莓多糖绿色合成纳米银颗粒的表征及其抗氧化活性研究 |
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引用本文: | 覃引,苟琴,熊音如,张群英,罗家豪,王锴楠. 树莓多糖绿色合成纳米银颗粒的表征及其抗氧化活性研究[J]. 信阳师范学院学报(自然科学版), 2019, 0(3): 453-458 |
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作者姓名: | 覃引 苟琴 熊音如 张群英 罗家豪 王锴楠 |
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作者单位: | 贵州理工学院食品药品制造工程学院;贵州大学生命科学学院;贵州省大健康医药产业技术研究院;贵州贵山红农业发展有限公司 |
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摘 要: | 从树莓果实中提取的树莓多糖和硝酸银反应合成多糖纳米银颗粒((PAgNPs),通过紫外-可见光(UV-Vis spectrum)全波段扫描、扫描电镜(SEM)以及X射线衍射(XRD)对其结构进行表征,并做抗氧化活性测试.结果表明,纳米银颗粒在435 nm处有最大吸收峰,经电镜检测多糖纳米银颗粒平均直径在20~32 nm.同时经抗氧化活性测试,对1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基半数清除浓度(IC_(50))分别为为64.38 mg/L和75.86 mg/L.
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关 键 词: | 树莓 多糖 纳米颗粒 质量表征 抗氧化活性 |
Characterization of Silver Nanoparticles Biosynthesized using Crude Polysaccharides of Rubus idaeus and Their Antioxidant Activities |
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Affiliation: | ,School of Pharmaceutical Engineering,Guizhou Institute of Technology,College of Life Sciences,Guizhou University,Guizhou Institute of Technology for Great Health Pharmaceutical Industry,Guizhou Guishanhong Agricultural Development Co.Ltd. |
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Abstract: | Silver nanoparticles(PAgNPs) were green synthesized using crude polysaccharides from Rubus idaeus.extracts and AgNO_3 solution. The obtained PAgNPs were characterized by UV-Vis absorption spectrum,scanning electron microscopy(TEM) and X-ray diffraction(XRD). Also,the antioxidant properties of the obtained PAgNPs were assessed in vitro,using the 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2'-azinobis(3-ethylbenzthiazino-6-sulphonic acid)(ABTS) radical cation decolorization test. The result indicates that the obtained PAgNPs regular spheres with an average size of 20 ~ 32 nm and a maximum absorbance at 435 nm. Moreover,PAgNPs exhibited a high radical scavenging activity for DPPH and ABTS+,half radical scavenging activities(IC_(50)) of 64.38 mg/L and 75.86 mg/L,respectively. |
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Keywords: | Rubus idaeus L. polysaccharide nanoparticles characterization antioxidant activitie |
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