序贯实验设计改进高效硝化菌发酵培养基

投加硝化菌菌剂是有效去除水体中亚硝酸盐的方法之一, 而作为商品化的硝化菌产品却非常少见.究其原因, 硝化菌的亚硝酸盐降解速率过低是重要的影响因素之一.本文对自然界筛选得到的硝化菌, 利用序贯实验设计（包括Plackett-Burmn Design、部分析因设计、最速上升法和部分组合设计等）优化得到一最佳培养基, 其组成（g/L）为：NaHCO3 1.86、NaNO2 2.04、Na2CO3 0.2、NaCl 0.2、KH2PO4 0.1、MgSO4 0.1和FeSO4 0.01.硝化菌的降解速率由初始580.7 mgNO2-N/gMLSS•d提高至859.5 mgNO2-N/gMLSS•d, 提高了48.2%.将菌体应用于模拟和真实养殖水体亚硝酸盐降解试验中，获得了成功.

Sequential Statistical Experimental Design Improves the Fermentation Medium for Nitrite Oxidizing Bacteria

The oxidation of nitrite to nitrate by nitrite oxidizing bacteria (NOB) in aquaculture systems was an important process, while it was difficult to get the commercial NOB in the market, the one of reasons was that the nitrite degraded rate of NOB was too low during the course of use. The sequential statistical experimental design (including Plackett-Burman Design, Factorial Design, Steepest ascent method and Central component design) was used to optimize the culture mediun of NOB, which isolated from nature. The optimal medium composition was found to be NaHCO3 1.86g/L, NaNO2 2.04g/L, Na2CO3 0.2g/L, NaCl 0.2g/L, KH2PO4 0.1g/L, MgSO4 0.1g/L and FeSO4 0.01g/L, the result of the nitrite degraded rate was improved from 580.7mgNO2-N/gMLSS•d to 859.5mgNO2-N/gMLSS•d, which increased by 48.2%. The results of simulated aquaculture and field trial of NOB show clearly that the NOB was effective at oxidizing nitrite to nitrate