| 端粒酶催化亚单位hTRT基因在人肝癌组织中的表达 |
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| 引用本文： | 曾常茜,刘英男,王振明,宿丽娟.端粒酶催化亚单位hTRT基因在人肝癌组织中的表达[J].北华大学学报(自然科学版),2003,4(4):304-305. |
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| 作者姓名： | 曾常茜 刘英男 王振明 宿丽娟 |
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| 作者单位： | 1. 北华大学,医学院,吉林,吉林,132001
2. 吉林化学工业集团公司总医院,吉林,吉林,132021 |
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| 摘 要： | 目的：探讨人端粒酶催化亚单位hTRT基因在肝癌组织中的表迭情况．方法：用兔抗人端粒酶催化亚单位多克隆抗体作为一抗，生物素化羊抗兔IgG作为二抗，对肝癌组织石蜡切片采用免疫组织化学方法测定hTRT基因的表达．结果：30例肝癌组织中25例检出hTRT阳性(25／30)，正常肝组织无l例阳性．结论：免疫组织化学法检测hTRT敏感性高、特异性强、重复性好，较其他方法简便、快速、经济且无放射性核素污染等缺点，临床具有推广价值．
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| 关 键 词： | 端粒酶 端粒酶催化亚单位 免疫组织化学方法 |
| 文章编号： | 1009-4822(2003)04-0304-02 |
| 修稿时间： | 2002年12月10日 |
Expression of the Catalytic Subunit Associated with Telomerase Gene in Human Liver Cancer |
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| ZENG Chang-qian,LIU Ying-nan,WANG Zhen-ming,SU Li-juan.Expression of the Catalytic Subunit Associated with Telomerase Gene in Human Liver Cancer[J].Journal of Beihua University(Natural Science),2003,4(4):304-305. |
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| Authors: | ZENG Chang-qian LIU Ying-nan WANG Zhen-ming SU Li-juan |
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| Abstract: | Objective To investigate human telomerase activity and clinical application by
immunohistochemistry assay. Methods Rabbit anti-human telomerase catalytic subunit polyclonal antibody was used specific antibody and sheep anti-rabbit IgG was used the second antibody. Paraffin slice samples of tumor tissues were detected by immunohistochemistry assay. Results Telomerase activity of 25 cases was positive in 30 cases of liver cancer tissues. The positive rate of telomerase activity was 25/30. Conclusion Immunohistochemistry assay for telomerase activity is sensitive, reproducible, simple, rapid, cheap and without radioisotopes pollution and damage. Therefor, it can be applied extensively clinically. |
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| Keywords: | Telomerase activity Telomerase catalytic subunit Immunohistochemistry assay |
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