应用傅立叶变换红外光谱研究白血病骨髓血红细胞

研究了急性粒细胞白血病(AML)和慢性粒细胞白血病(CML)患者的骨髓血红细胞的红外光谱差异性：与CML红细胞相比，AML红细胞酰胺Ⅰ带较宽；1540cm-1与1 084cm-1吸收峰面积之间的比值较低，范围较窄。该结果提示AML和CML骨髓血红细胞系中有核红细胞的数量分布不同，同时提示骨髓红细胞的成熟可能与白血病细胞的增殖有关。骨髓血细胞的红外光谱的差异性对白血病的分析和评价具有重要的意义。

Fourier Transform Infrared Spectroscopic Investigation of Human Erythrocytes in Bone Marrow

Eight samples of erythrocytes in bone marrow coming from patients affected by acute myeloid leukemia (AML) and nine samples from patients suffered from chronic myeloid leukemia (CML) were studied by FTIR spectroscopy.Spectroscopic differences of leukemic erythrocytes were observed between AML and CML samples.The ratios of the integrated areas of the band at 1540 cm-1 due to the protein components of erythrocytes,and of the band from 1084 cm-1 mainly involving the vibration of the O-P-O linkages and C-O groups in ribosome,are closer and lower in AML samples than the corresponding ratios in CML samples.The amide Ⅰ　band in AML is broader than that in CML.These differences indicate the different contributions of red cells in erythrocytic series in the erythropoietic development from proerythroblast to matured erythrocytes,and the results imply the maturation of red cells in bone marrow are related to the proliferation of leukemic cells.The noticeable differences may have important implications for analyzing and evaluating leukemia.