(1) The National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China;(2) The Graduate School of Chinese Academy of Sciences, 100039 Beijing, China
Abstract:
The purified thermophilic bacterium PS3 F1β10 ×His-tag is inserted into the F0F1-ATP synthases of chromatophores isolated from photosynthetic bacteria Rhodospirillum rubrum. The studies of biochemical properties of the hybrid chromatophores show that they have both protonsdriving capability and photophosphorylation. The fluorescent actin filaments, as a marker of its orientation by videomicroscopic experiment, are connected via Maleimido-C3-NTA to the reconstituted β10 ×Histag of F0F1ATP synthases. The clockwise rotation of F0F1ATP synthases driven by light is observed directly when viewed from the F0 side to F1. This system should be valuable for further studying the coupling property of F0F1ATP synthase.
