| Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells |
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| 作者姓名： | CHEN Zhiling YU Yi LIU Lin XIA Guixian |
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| 作者单位： | 1. College of Life Science, Capital Normal University, Beijing 100037, China; 2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
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| 基金项目： | Supported by the National Basic Research Priorities Program (Grant No. 2002CCA03000)，National Natural Science Foundation of China (Grant No. 30100091) |
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| 摘 要： | The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48. The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245?374 and aa 518?646). Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through G1 to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells.
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| 关 键 词： | tobacco BY-2 suspension cells NtCDC48 spindle cell cycle. |
Isolation, characterization and functional analysis of a cdc48 homologue from tobacco BY-2 cells |
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| Authors: | CHEN Zhiling YU Yi LIU Lin XIA Guixian |
| |
| Institution: | 1. College of Life Science, Capital Normal University, Beijing 100037, China; 2. Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | The fission yeast Schizosaccharomyces pombe was used to identify genes from tobacco BY-2 cells that may play roles in cell cycle regulation. A cDNA encoding a protein homologous to the yeast CDC48 was isolated and the gene was designated as NtCDC48. The cDNA contains an open reading frame coding for a predicted protein of 808 amino acids which comprises of two typical ATPase modules (aa 245?374 and aa 518?646). Overexpression of NtCDC48 in tobacco BY-2 cells led to an increase in the mitotic index as well as to the formation of diffused mitotic spindles. NtCDC48-GFP fusion proteins are distributed ubiquitously through G1 to M phases, yet their subcellular localization varied regularly along with the cell cycle progression. These results indicate that NtCDC48 may play an important role in the regulation of cell cycle in BY-2 cells. |
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| Keywords: | tobacco BY-2 suspension cells NtCDC48 spindle cell cycle |
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