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毒死蜱降解菌CD7的筛选与植物根际促生菌JD37的联合应用
引用本文:苏翠珠,蒋秋悦,章丽,罗斯,杨文武,孙舒荣,肖明.毒死蜱降解菌CD7的筛选与植物根际促生菌JD37的联合应用[J].上海师范大学学报(自然科学版),2017,46(2):311-318.
作者姓名:苏翠珠  蒋秋悦  章丽  罗斯  杨文武  孙舒荣  肖明
作者单位:上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234,上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234;复旦大学 生命科学学院, 上海 200438,上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234,上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234,上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234,上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234,上海师范大学 生命与环境科学学院 植物种质资源开发协同创新中心, 上海 200234
基金项目:上海市科委重点项目(16391902100)
摘    要:筛得一株毒死蜱降解菌CD7,联合植物根际促生菌JD37,研制了一种能修复土壤的农药污染,同时促进植物生长的复合型土壤改良剂.实验结果显示:将CD7和JD37发酵液按体积比1∶1使用,可以促进植物生长,并在25 d内降解土壤中约66.43%的毒死蜱.在载体量相同情况下,蚯蚓粪比滑石粉吸附更多混合菌株.在室温贮藏一个月,活菌数为4.81×10~7CFU/g;制备的复合改良剂与土壤按质量比1∶1使用时,能促进植物生长、提升土壤酶活性、增加土壤中微生物的数量.

关 键 词:植物根际促生菌  毒死蜱降解菌  CD7  土壤改良  筛选
收稿时间:2016/10/31 0:00:00

Screening of chlorpyrifos degrading bacteria CD7 and its combined application with PGPR JD37
Su Cuizhu,Jiang Qiuyue,Zhang Li,Luo Si,Yang Wenwu,Sun Shurong and Xiao Ming.Screening of chlorpyrifos degrading bacteria CD7 and its combined application with PGPR JD37[J].Journal of Shanghai Normal University(Natural Sciences),2017,46(2):311-318.
Authors:Su Cuizhu  Jiang Qiuyue  Zhang Li  Luo Si  Yang Wenwu  Sun Shurong and Xiao Ming
Institution:Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China;School of Life Sciences, Fudan University, Shanghai 200438, China,Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China,Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China and Development Center of Plant Germplasm Resources, College of Life and Environmental Sciences, Shanghai Normal University, Shanghai 200234, China
Abstract:We screened a chlorpyrifos degrading bacteria,Burkholderiasp. CD7.Joint with plant growth-promoting rhizobacteria(PGPR) JD37 to produce a compositesoil amendment,which could restorethe pesticides polluted soil and promote plant growth.Results showed that CD7 and JD37 (at the volume ratio of 1:1) can promote the growth of plants,and within 25 days degrade about 66.43% chlorpyrifos in the soil.Further research found that under the same conditions of carrier dosage,vermicompost can adsorbed more bacteria than talcum powder;after a month preservation at room temperature,the number of living bacterium still maintained about 4.81×107 CFU/g.Carrier and soil,at the mass ratio of 1:1,could optimally promote plant growth,improve soil enzyme activities and increase the number of microorganisms in soil.
Keywords:plant growth-promoting rhizobacteria  chlorpyrifos degrading bacteria CD7  soil amendment  screening
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