| 苔藓植物DNA不同提取方法的比较分析 |
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| 引用本文： | 张安世,邢智峰,刘永英,张为民.苔藓植物DNA不同提取方法的比较分析[J].河南科学,2009(5). |
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| 作者姓名： | 张安世 邢智峰 刘永英 张为民 |
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| 作者单位： | 焦作师范高等专科学校生物系,河南,焦作,454001 |
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| 基金项目： | 河南省科技攻关项目，河南省教育厅自然科学研究计划项目 |
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| 摘 要： | 以苔藓植物为材料,比较快速提取法、SDS法和改良CTAB法(自行设计)提取苔藓植物总DNA的效果.结果表明:改良CTAB法提取的基因组DNA质量最好(A260/A280为1.83～1.86;A260/A230为2.05～2.50),快速提取法次之(A260/A280为1.64～1.73;A260/A230为1.68～1.77),SDS法较差(A260/A280为1.51～1.61;A260/A230为1.56～1.76).改良CTAB法提取的苔藓植物的DNA适合于作为PCR的模板,并成功地进行了RAPD扩增.
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| 关 键 词： | 苔藓植物 DNA提取 基因组DNA SDS法 CTAB法 RAPD扩增 |
Comparison and Analysis on the Different Methods of DNA Extration Methods from Bryophytes |
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| Zhang Anshi,Xing Zhifeng,Liu Yongying,Zhang Weimin.Comparison and Analysis on the Different Methods of DNA Extration Methods from Bryophytes[J].Henan Science,2009(5). |
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| Authors: | Zhang Anshi Xing Zhifeng Liu Yongying Zhang Weimin |
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| Institution: | Department of Biology;Jiaozuo Teachers College;Jiaozuo 454001;Henan China |
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| Abstract: | With Bryophytes used as ingredient,to compare total DNA extracted effects of the different three methods such as the fast extraction,SDS and the improved CTAB method which was designed by our research group.The results showed that the improved CTAB method had a remarkable superiority overothers(A260/A280 was 1.83～1.86;A260/A230 was 2.05～2.50),followed by the fast extraction(A260/A280 was 1.64～1.73;A260/A230 was 1.68～1.77)and the worst was SDS(A260/A280 was1.51～1.61;A260/A230 was1.56～1.76).The DNA was extracted by using improved CTAB method was suitable for PCR as a template,and successful for PAPD amplification. |
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| Keywords: | bryophytes DNA extraction genomic DNA SDS method CTAB mehood PAPD amplification |
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