金属离子对盆栽银杏叶PAL酶活及黄酮含量的影响

对6年生盆栽银杏苗分别喷施4种金属盐诱导其叶片苯丙氨酸解氨酶(PAL)活性,紫外分光光度计测定银杏叶2002年的黄酮含量和PAL活性年周期变化,以及不同处理下银杏叶PAL活性.结果表明:4种金属盐处理对银杏叶中PAL活性及黄酮含量有显著的影响,从9月19日开始,分别用0.1%、0.3%的Fe-SO4、0.1%、0.2%、0.3%的ZnSO4,0.1%、0.3%、0.5%的MnSO4和0.1%、0.4%的CuSO4处理,不仅可提高叶黄酮含量,而且还延长了最佳采叶期,其中提高黄酮含量最佳处理组合为0.1?SO4 0.2% ZnSO4 0.3%MnSO4 0.4%CuSO4,该处理下,叶片黄酮含量高达1.619 4%.

Effects of Some Metal Ions on Phenylalanine Ammoni-alyase Activities and Flavonoids Content of Ginkgo biloba Leaves in the Potted Orchard

The enzyme activities of PAL from six year old Ginkog biloba leaves in the potted orchard were induced by four groups of metal ions respectively. The PAL activities and flavonoids content were measured using the UV-Vis spectrophotometer. Changes of the whole year were also studied. The results showed that four groups of metal ion treatments had significant effects on the PAL activities and flavonoids contents in Ginkgo leaves. The treatments with 0.1% and 0.3 % FeSO4 , 0.1%. 0.2 % and 0.3 % ZnSO4 , 0.1%. 0.3 % and 0.5 % MnSO4, 0.1% and 0.4% CuSO4 not only enhanced flavonoids contents, but also extended the periods of best-collecting-leaf. The optimum combination was 0.1% FeSO4,0.2% Zn- SO4, 0. 3% MnSO4, 0.4% CuSO4. It could improve flavonoids content up to 1. 619 4%.