| 粪鬼伞多糖的提纯与定性研究 |
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| 引用本文： | 刘洪霞,田文莹,王志兵,邱芳萍.粪鬼伞多糖的提纯与定性研究[J].吉林工学院学报,2009,30(2):126-129. |
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| 作者姓名： | 刘洪霞 田文莹 王志兵 邱芳萍 |
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| 作者单位： | 长春工业大学化学与生命科学学院,吉林,长春,130012
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| 基金项目： | 吉林省教育厅科技计划项目 |
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| 摘 要： | 采用水提醇沉法提取粪鬼伞粗多糖（CPS），并对其提纯与鉴定。CPS经酶法结合Sevag法脱除蛋白，上DEAE—cellulose-52柱和Sepharose-6B凝胶柱层析，分离纯化得多糖CPS—Ⅰa。经紫外光谱扫描，Sephadex G-100凝胶柱层析和红外光谱分析，结果显示，多糖CPS—Ⅰa具有葡萄糖醛酸的特征吸收峰，α—D吡喃糖苷键连接，是一种酸性多糖。
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| 关 键 词： | 粪鬼伞多糖 纯化 定性 |
Purification of polysaccharides from Coprinus sterqulinus Fr. |
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| LIU Hong-xia,TIAN Wen-ying,WANG Zhi-bing,QIU Fang-ping.Purification of polysaccharides from Coprinus sterqulinus Fr.[J].Journal of Jilin Institute of Technology,2009,30(2):126-129. |
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| Authors: | LIU Hong-xia TIAN Wen-ying WANG Zhi-bing QIU Fang-ping |
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| Institution: | School of Chemical & Life Science;Changchun University of Technology;Changchun 130012;China |
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| Abstract: | With water-ethyl alcohol extraction method, crude Coprinus sterqulinus Fr. Polysaccharide （CPS） is obtained and then purified. CPS is united with Sevag to remove the proteins, upper DEAE-52 column and Sepharose-6B gel to get the component CPS-Ⅰ a. Sephadex G-100 is scanned with UV spectra and then analyzed with IR spectra, and the results indicate that CPS- Ⅰ a, as an acid polysaccharide, has an absorption peak for glucuronidase and α-D glycosidic bond. |
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| Keywords: | Coprinus sterqulinus Fr Polysaccharide purification extraction |
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