| 优质肉鸡ADSL及GART基因PCR-SSCP分析 |
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| 引用本文： | 王康环,蒋利,任冰冰,等. 优质肉鸡ADSL及GART基因PCR-SSCP分析[J]. 西南民族学院学报(自然科学版), 2013, 0(6): 851-854 |
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| 作者姓名： | 王康环 蒋利 任冰冰 等 |
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| 作者单位： | [1]西南民族大学生命科学与技术学院,成都610041 [2]四川省畜牧科学研究院,成都610066 |
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| 基金项目： | 四川省应用基础研究重点项目07JY029-045;西南民族大学创新型科研项目CX2013SZ58 |
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| 摘 要： | 采用PCR-SSCP技术对大恒鸡S01品系共计300只鸡的ADSL及GART基因进行多态性分析,结果显示：ADSL基因第9个外显子存在一个SNP位点,测序结果显示其纯合子CC与TT相比有一个C10191T的单碱基突变;GART基因在5’侧翼序列存一个SNP位点,其纯合子AA与BB相比有一个C153T单碱基突变,采用独立性x2分析发现,ADSL及GART基因都达到Hardy-Weinberg平衡.
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| 关 键 词： | IMP ADSL基因 GART基因 PCR-SSCP |
Analysis of PCR-SSCP on ADSL gene and GART gene of quality chicken |
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| WANG Kang-huan,JIANG Li,REN Bing-bing,LIU Guang-wei,JIANG Xiao-song,XU Ya-ou. Analysis of PCR-SSCP on ADSL gene and GART gene of quality chicken[J]. Journal of Southwest Nationalities College(Natural Science Edition), 2013, 0(6): 851-854 |
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| Authors: | WANG Kang-huan JIANG Li REN Bing-bing LIU Guang-wei JIANG Xiao-song XU Ya-ou |
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| Affiliation: | 1. School of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, P.R.C.; 2. Sichuan Animal Science Academy, Chendu 610066, P.R.C.) |
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| Abstract: | The polymorphisim of ADSL and GART gene in 300 chicken from Daheng S01 population by means of PCR-SSCP. The result shows that ADSL gene has one SNP in exon 9, GART gene has one snp 5＇UTR. The sequencing results indicate that CC genotypes have only one base difference with TT genotypes C2132T, while AA genotype has only one base difference with BB genotype C2132A. The result of Chi Square （χ2） test shows that the ADSL and GART gene reach Hardy-Weinberg. |
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| Keywords: | IMP ADSL gene GART gene PCR-SSCP |
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