| 高效液相色谱法测定畜禽肉中磷脂酰胆碱的含量 |
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| 引用本文： | 张云光,卢安根,莫建光,徐慧.高效液相色谱法测定畜禽肉中磷脂酰胆碱的含量[J].广西科学院学报,2010,26(3):242-244. |
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| 作者姓名： | 张云光 卢安根 莫建光 徐慧 |
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| 作者单位： | 1. 南宁邦尔克生物技术有限责任公司,广西南宁,530003
2. 广西分析测试研究中心,广西南宁,530022 |
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| 基金项目： | 广西大型仪器协作共用网资助课题 |
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| 摘 要： | 应用高效液相色谱法(HPLC)测定畜禽肉中磷脂酰胆碱含量。以Intertsil SIL硅胶柱(4.6mm×250mm,5μm)为分离柱,甲醇∶水(85∶15,V∶V)为流动相等度洗脱,流速1.5ml/min,柱温为室温,用紫外检测器在206nm处对磷脂酰胆碱进行测定。测得磷脂酰胆碱在0.5～1.92 mg/ml范围内线性关系良好(r2=0.9957),方法检测限为4.14μg/g,精密度为0.49%(n=5),回收率为89%～102%。该方法灵敏度高,重现性和稳定性比较好。
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| 关 键 词： | 高效液相色谱法 畜禽肉 磷脂酰胆碱 含量测定 |
| 收稿时间： | 2010/5/12 0:00:00 |
Determination of Phosphatidyl Choline in Meat by High Performance Liquid Chromatography |
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| ZHANG Yun-guang,LU An-gen,MO Jian-guang and XU Hui.Determination of Phosphatidyl Choline in Meat by High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2010,26(3):242-244. |
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| Authors: | ZHANG Yun-guang LU An-gen MO Jian-guang and XU Hui |
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| Institution: | 1.Nanning Bioclone Biotechnology Co.,Ltd.,Nanning,Guangxi,530003,China;2.Guangxi Research Center of Analysis and Testing,Nanning,Guangxi,530022,China) |
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| Abstract: | The phosphatidyl choline（PC） in meat is determinated using high performance liquid chromatography（HPLC）.Phosphatidyl choline was successfully separated on a Intertsil SIL column（4.6mm×250mm,5μm）using methanol-water（85∶15,V∶V）as mobile phase at a flow rate of 1.5ml/min and the drift tube temperature is room-temperature.The linear detection range of PC was 0.5～1.92 mg/ml（r2=0.9957） and the limit of etection（LOD） was 4.14μg/g with excellent relative standard deviation（RSD） of 0.49%（n=6）.The recovery of PC was in the range of 89%～102%.These results indicated that this method was a simple and fast method with more accuracy and reproduciblity to detect phosphatidyl choline. |
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| Keywords: | HPLC meat phosphatidyl choline quanlity determination |
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