Detection of human fibrin by immunological piezoelectric quartz crystal microbalance technique |
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Authors: | Pei Renjun Hu Jiming Hu Yi Zeng Yun’e |
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Institution: | (1) Department of Chemistry, Wuhan University, 430072 Wuhan, China;(2) Department of Analysis-Measurement Science, Wuhan University, 430072 Wuhan, China |
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Abstract: | The detection of human fibrin has been performed by immunological piezoelectric quartz crystal microbalance technique. Anti-fibrin
antibody was immobilized onto the gold electrodes of a 9 MHz AT-cut piezoelectric quartz crystal with bovine serum albumin
adsorption, followed by glutaraldehyde cross-linking. The antibody-bound crystals had good response to human fibrin in the
concentration range of 10−4∼10−1g/L. The sensor system had good selectivity. The crystal could be regenerated nearly 10 times without detectable decrease
of sensitivity.
Supported by The National Natural Science Foundation of China
Pei Renjun: born in Nov. 1971, Ph. D. graduate student |
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Keywords: | human fibrin quartz crystal microbalance antibody immobilization regeneration |
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