Cloning, sequencing and polymorphism analyzing of the exon 4 of the kappa-casein gene in yak |
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| Authors: | FAN Baoliang LI Ning HU Xiaoxiang WU Changxin |
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| Institution: | Hebei Handan Agricultural college, Handan 057150, China,State Key Laboratories for Agrobiotechnology,College of Animal Science and Technology, China Agricultural University, Beijing 100094, China,College of Animal Science and Technology, China Agricultural University, Beijing 100094, China |
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| Abstract: | According to the nucleotide sequence of cattle kappa- casein gene, a pair of primers was synthesized
to amplify the sequences of the exon 4 and partial intron 4 of this gene in yak. The restriction fragment length polymor¬phisms were identified by the digestions of the PCR product with Hind 111 or Pst I in both populations of cattle and yak, 'but the frequency of the allele and the genotype were significantly different between the two animal populations. |
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| Keywords: | yak kappa-casein gene exon 4 polymorphism |
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