| 组成型表达CsCOR1基因烟草的制备 |
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| 引用本文： | 李先文,李铃,李铮铮,张新玉.组成型表达CsCOR1基因烟草的制备[J].信阳师范学院学报(自然科学版),2012(1):64-66. |
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| 作者姓名： | 李先文 李铃 李铮铮 张新玉 |
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| 作者单位： | 信阳师范学院生命科学学院;信阳市五星医院 |
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| 基金项目： | 河南省科技攻关项目(082102150009);信阳师范学院博士启动基金项目 |
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| 摘 要： | 将已克隆的一个被冷显著上调的基因CsCOR1整合到穿梭载体pBI121的CaMV35S启动子的下游,然后采用农杆菌介导法转化进烟草细胞,从而制得了转CsCOR1基因烟草植株,并筛选到组成型表达CsCOR1基因的烟草转化子,为进一步研究该基因的功能奠定了基础.
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| 关 键 词： | 表达载体构建 遗传转化 烟草转化子 超表达 |
Preparation of Tobacco Transformants with Overexpression CsCOR1 Gene |
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| LI Xian-wen,LI Ling,LI Zheng-zheng,ZHANG Xin-yu.Preparation of Tobacco Transformants with Overexpression CsCOR1 Gene[J].Journal of Xinyang Teachers College(Natural Science Edition),2012(1):64-66. |
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| Authors: | LI Xian-wen LI Ling LI Zheng-zheng ZHANG Xin-yu |
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| Institution: | 1(1.College of Life Sciences,Xinyang Normal University,Xinyang 464000,China; 2.The Wuxing hospital of Xinyang City,Xinyang 464000,China) |
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| Abstract: | CsCOR1,a gene upregulated remarkably by cold,was inserted CaMV35S promoter behind of pBI121 vector,producing a pBI121-CsCOR1.Then,the vector was introduced Agrobacterium tumefaciens strain EHA105 using freeze-thaw transformation.Transgenic-tobaccos were generated by Agrobacterium-mediated transformation using the leaf disc system,and two plants overexpressing CsCOR1 gene were screened.The harvests lay the foundation of CsCOR1 function research. |
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| Keywords: | constructing expression vector genetic transformation tobacco transformants overexpression |
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