| 荧光光度法结合固相萃取测定亚硝酸盐 |
| |
| 引用本文： | 黄克靖,孙俊永,吴莹莹.荧光光度法结合固相萃取测定亚硝酸盐[J].信阳师范学院学报(自然科学版),2012(1):99-102. |
| |
| 作者姓名： | 黄克靖 孙俊永 吴莹莹 |
| |
| 作者单位： | 信阳师范学院化学化工学院 |
| |
| 基金项目： | 河南省高校科技创新人才基金项目(2010HASTIT025);河南省科技创新杰出青年基金项目(104100510020) |
| |
| 摘 要： | 以石墨烯为吸附剂制备固相萃取柱,研究影响萃取柱萃取效率的因素.以8-(3’,4’-二氨基苯)-二氟化硼-二吡咯甲烷为荧光探针,在30℃、酸性条件下,与亚硝酸根反应15 min后生成荧光衍生产物.衍生产物经固相萃取后用荧光光度法进行测定,建立了一种荧光光谱法灵敏测定亚硝酸盐的新方法.方法的线性范围为0.2～200 nmol/L.当信噪比等于3时,方法的检出限为0.08 nmol/L.将该方法用于食品中亚硝酸盐的测定,加标回收率为94.0%～110.4%.
|
| 关 键 词： | 荧光光度法 石墨烯 亚硝酸盐 食品 |
Determination of Nitrite by Spectrofluorimetry with Solid-phase Extraction |
| |
| HUANG Ke-jing,SUN Jun-yong,WU Ying-ying.Determination of Nitrite by Spectrofluorimetry with Solid-phase Extraction[J].Journal of Xinyang Teachers College(Natural Science Edition),2012(1):99-102. |
| |
| Authors: | HUANG Ke-jing SUN Jun-yong WU Ying-ying |
| |
| Institution: | (College of Chemistry and Chemical Engineering,Xinyang Normal University,Xinyang 464000,China) |
| |
| Abstract: | An efficient solid-phase extraction column was fabricated using graphene as adsorbent.Some factors affecting the extraction efficiency were studied.Fluorescent production was produced after 8-(3’,4’-diaminophenyl)-difluoroboradiaza-s-indacene as fluorescent probe reacted with nitrite in aqueous medium at 30 ℃ for 15 min.A new spectrofluorimetry method was developed for the sensitive determination of nitrite after extracting nitrite derivative.The response was a linear function of concentration in the range of 0.2~200 nmol/L.The detection limit was calculated to be 0.08 nmol/L(RSN=3).The proposed method was used to determine the content of nitrite in food samples with the recoveries of 94.0%~110.4%. |
| |
| Keywords: | spectrofluorimetry graphene nitrite food samples |
| 本文献已被 CNKI 等数据库收录！ |
