浙江清凉峰地衣内生菌物种多样性及活性研究

采用挑取地衣体髓层菌丝法对浙江省清凉峰的地衣内生菌进行分离培养。基于ITS序列和28S序列的系统发育分析表明，选取的55株内生菌都属于子囊菌门且具高度的物种多样性。用滤纸片法检测该内生菌次生代谢产物的抗细菌、真菌活性，发现其中有8株具抗菌活性，占所测菌株的14.55%，抑菌圈的直径最大达到3 cm。研究表明地衣内生菌可产生丰富的具抑菌活性的次生代谢产物，可作为对多种病原物具有抑菌活性物质筛选的资源，是抑菌活性物质潜在的、丰富的资源库。

Species diversity and antibiological activities of endolichenic fungi from Zhejiang Mt.Qingliang

We directly selected medulla mycelium of the lichen to culture the endolichenic fungi from Zhejiang Mt. Qingliang. The ITS nrDNA and 28S nrDNA sequences based growth analysis indicates that 55 fungi belong to Ascomycota. We determined the bacteriostatic activity of secondary metabolite of these 55 endolichenic fungi by the method of paper disc. Results show that 14.55% of the total strains (8 strains) have highly antifungal activity. Maximum diameter of filter parer piece is 3 cm. Our research demonstrates that endolichenic fungi can produce abundant secondary metabolites with antimicrobial activity. They can be served as good resources to screen active substances for many pathogens. They form a potential and rich resource library of antimicrobial compounds.