| 丹参嫩茎组织培养及无性系建立的研究 |
| |
| 引用本文： | 徐娜,宁淑香,张美燕.丹参嫩茎组织培养及无性系建立的研究[J].辽宁大学学报(自然科学版),2011,38(3):214-218. |
| |
| 作者姓名： | 徐娜 宁淑香 张美燕 |
| |
| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116029 |
| |
| 摘 要： | 为保护濒危植物丹参,实现人工栽培,以嫩茎为材料,进行了愈伤组织诱导与分化、试管苗生根、继代、移栽和定植研究.结果证明:MS+6-BA0.3 mg.L-1+2,4-D2.0 mg.L-1+NAA0.8 mg.L-1是丹参嫩茎愈伤组织诱导培养和继代培养的理想培养基;1/2MS+AgNO30.2 mg.L-1和1/2MS+AgNO30.2 mg.L-1+6-BA 0.1 mg.L-12种培养基是丹参颗粒状愈伤组织块分化培养的理想培养基;以经过浓度为10mg.L-1的NAA溶液处理3 m in的不定芽为材料、以1/3MS+IAA0.4 mg.L-1为培养基的方法是丹参不定芽生根培养的理想方法.试管苗移栽成活率为92.7%,定植成活率为99.2%.定植的试管苗保持了野生丹参的所有生物性,建立起丹参的无性系.
|
| 关 键 词： | 丹参 组织培养 无性系 |
Study on Tissue Culture and Establishment of Salvia miltiorrhiza Clone |
| |
| XU Na
,
NING Shu-xiang
,
ZHANG Mei-yan.Study on Tissue Culture and Establishment of Salvia miltiorrhiza Clone[J].Journal of Liaoning University(Natural Sciences Edition),2011,38(3):214-218. |
| |
| Authors: | XU Na NING Shu-xiang ZHANG Mei-yan |
| |
| Institution: | XU Na,NING Shu-xiang,ZHANG Mei-yan(College of Life Science,Liaoning Normal University,Dalian 116029,China) |
| |
| Abstract: | The tender stem was used as materials to establish the rapid propagation technique of Salvia miltiorrhiza such as the calli induction and differentiation,rooting and transplanting of tube seedlings and so on.The results showed that the optimum culture medium for calli induction and subculture was MS+6-BA0.3 mg L-1+2,4-D2.0mg L-1+NAA0.8mg L-1;1/2MS+AgNO30.2mg L-1 and 1/2MS+AgNO30.2mg L-1+6-BA 0.1mg L-1 were the two kinds of optimum culture medium for differentiation of grainy calli induction.The adventitious... |
| |
| Keywords: | Salvia miltiorrhiza tissue culture clone |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
