| rh—bFGF抑制顺铂诱导的人胃腺癌BGC—823细胞凋亡 |
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| 引用本文： | 辛洪启,林剑,洪岸,袁守军,汤仲明.rh—bFGF抑制顺铂诱导的人胃腺癌BGC—823细胞凋亡[J].暨南大学学报,2001,22(1):120-124. |
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| 作者姓名： | 辛洪启 林剑 洪岸 袁守军 汤仲明 |
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| 作者单位： | 1. 暨南大学生物工程研究所
2. 军事医学科学院放射医学研究所 |
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| 基金项目： | 国家“九五”重点攻关资助!项目 (96 -C0 2 - 0 1- 0 3) |
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| 摘 要： | 目的：评价外源加入的rh-bFGF对抗肿瘤药物顺铂所致人胃腺癌细胞凋亡的影响。方法：利用顺铂诱导人胃腺癌BGC-823细胞产生的凋亡作模型，体外培养时在顺铂作用前或作用后加入不同浓度的rh-bFGF，观察细胞形态的变化和细胞凋亡经率的变化。结果：预培养的rh-bFGF质量浓度为10ng/mL及1ng/mL时抑制细胞凋亡的发生，共培养时则在质量浓度为1ng/mL时产生抑制调亡的作用。结论：外源加入的rh-bFGF在一定的浓度范围内，不信纸预培养还是与顺铂的共培养12h，均可不同程度的抑制顺铂胃腺癌BGC-823细胞的凋亡，并且这种剂量效应与rh-bFGF影响BGC-823细胞的细胞周期相关。
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| 关 键 词： | 重组人碱性成纤维细胞生长因子 rh－bFGF 顺铂 人胃腺癌 BGC－823细胞 细胞凋亡 药物抗性 |
| 文章编号： | 1000-9965(2001)01-0120-05 |
| 修稿时间： | 2000年11月6日 |
The inhibitson of cisplatin-induced apoptosis in BGC-823 human stomach cancer cells by basic fibroblast growth factor |
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| XIN Hong-qi ,YUAN Shou-jun ,\ TANG Zhong-ming ,\ LIN Jian ,HONG An.The inhibitson of cisplatin-induced apoptosis in BGC-823 human stomach cancer cells by basic fibroblast growth factor[J].Journal of Jinan University(Natural Science & Medicine Edition),2001,22(1):120-124. |
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| Authors: | XIN Hong-qi YUAN Shou-jun \ TANG Zhong-ming \ LIN Jian HONG An |
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| Institution: | XIN Hong-qi 1,YUAN Shou-jun 2,\ TANG Zhong-ming 2,\ LIN Jian 1,HONG An 1 |
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| Abstract: | Aim: To evaluate the suppression of the apoptosis induced by cisplatin in BGC-823 human stomach cancer cells by rh-bFGF. Methods: Using the apoptosis model in BGC-823 human stomach cancer cells cisplatin induced, we studied the change of cytomorphology and the rate of apoptosis cell when pre-cultue or co-culture with recombined human basic fibroblast growth factor(rh-bFGF) in vitro . Results: rh-bFGF can inhibit cisplatin-induced apoptosis in BGC-823 human stomach cancer cells either pre-culture in 12 hours at 1 ng/mL and 10 ng/mL, or co-culture with cisplatin in 12 hours at 1ng/mL. Conclusion: rh-bFGF can inhibit the apoptosis in BGC-823 human stomach cancer cells induced by cisplatin at some concentration range in vitro , it maybe associate with the effect that rh-bFGF influence the cell cycle. |
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| Keywords: | recombined human fibroblast growth factor(rh-bFGF) cisplatin \ apoptosis |
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