H2O2诱导玉米根尖细胞凋亡的形态,生化及分子生物学证据

植物细胞程序性死亡是近年来植物细胞生物学的研究热点之一。已有证据表明Ｈ２Ｏ２能诱导拟南芥、豌豆，烟草等植物体外培养细胞的凋亡。以玉米根尖为材料，从原位即细胞和染色体水平（染色体ＤＮＡ断裂）以及分子水平（ＤＮＡ）对Ｈ２Ｏ２诱导的细胞死亡作了检测。结果表明Ｈ２Ｏ２能诱导玉米根尖细胞凋亡，并具有动物细胞凋亡的典型形态和生化特征如染色质凝缩，核降解以及出现ＤＮＡ Ｌａｄｄｅｒ等。可见Ｈ２Ｏ２诱导的细胞凋亡

MORPHOLOGICAL BIOCHEMICAL AND MOLECULAR EVIDENCES FOR H_2O_2 - INDUCED APOPTOSIS IN CELLS OF MAIZE ROOTS

The investigation of programmed cell death in plants has blossomed in recent years inplant cytohiology. There is increasing evidence that H2O2 can induce apoptosis in vitro in various plantspecies such as Arabidopsis, pea and tobacco. We provide morphological and biochemical and moecularevidences for specific apoptotic changes in maize induced by H2O2 with a simple and high - efficient chromosome spreading - based TUNEL assay and DNA Laddering. The results show that H2O2 can induceoligomeric ladders and charecteristic chromtin/chromosom condensation, similar to those in animalapoptosis. It can be concluded that H2O2 - induced apoptosis is ubiquitous in plants.