茉莉酸甲酯对杂交鹅掌楸体胚发育的影响

【目的】探讨作为植物生长调节剂的茉莉酸甲酯(Me JA)对杂交鹅掌楸体胚发育的影响,为进一步提高杂交鹅掌楸体胚发生效率奠定基础。【方法】在已建立的杂交鹅掌楸未成熟胚体细胞发生体系的基础上,选用杂交鹅掌楸3种基因型C138、233012、253010为材料,分别添加5种浓度的茉莉酸甲酯,测定杂交鹅掌楸在添加不同浓度茉莉酸甲酯条件下的体胚发生率、成熟率、畸形胚发生率,以及再生植株率。【结果】在体胚诱导培养基中添加不同浓度的Me JA可以显著影响杂交鹅掌楸体胚发生效率,且随着Me JA浓度的增加,体胚发生率和成熟率逐渐升高,畸形胚发生率逐渐降低,而当Me JA浓度超过一定值后,体胚发生率和成熟率呈现下降趋势,畸形胚发生率也逐渐升高。杂交鹅掌楸3种基因型体胚发生率和成熟率从高到低的次序为233012、C138、253010。脱落酸(ABA)能够增强Me JA的上述效应。在体胚苗的生长阶段,浓度逐渐升高的Me JA限制了根的生长,但ABA不能改变该效应。【结论】添加1μmol/L Me JA和2 mg/L ABA时杂交鹅掌楸体胚诱导效果最佳,此时体胚发生率和体胚成熟率相对较高,体胚畸形率最低。

Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid

Object] In order to further increase the frequency of somatic embryogenesis, the experiment was conducted to investigate the effect of methyl jasmonate ( MeJA) on somatic embryogenesis development of Liriodendron hybrid.Methods]Based on an established system of somatic embryogenesis of Liriodendron hybrid, embryogenic cells of three genotypes included C138, 233012 and 253010 from Liriodendron chinense×L.tulipifera were cultured in the embryo in-duction medium with different concentrations of MeJA, and the rates of somatic embryogenesis, somatic embryo matura-tion, abnormal somatic embryos, and number of regeneration plants were investigated. Result] The different concentra-tions of MeJA can significantly affect the efficiency of Liriodendron hybrid somatic embryogenesis, and with the increase of MeJA concentration, the rates of somatic embryogenesis and maturation gradually increased, the rate of abnormal em-bryos decreased gradually. However, when the MeJA concentration exceeds a certain value, the rates of somatic embryo-genesis and maturation showed a decreased trend, and the number of abnormal embryos also increased. Of which geno-type 233012 was in the first place as well as genotype C138 was higher than 253010. Abscisic acid (ABA) could en-hance the above-mentioned effects of MeJA. But the increasing concentration of MeJA restricted the root growth, and ABA could not alter this effect. Conclusion]The induction effect of Liriodendron hybrid somatic embryos could be opti-mal when adding 1 mol/ L MeJA and 2 mg/ L ABA, the rates of somatic embryogenesis and somatic embryo maturation were relatively high, with the lowest rate of abnormal embryos.