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动态载片培养法批量制作永久细菌标本片
引用本文:杨冬华,杨君玲. 动态载片培养法批量制作永久细菌标本片[J]. 实验科学与技术, 2012, 10(4): 26-29
作者姓名:杨冬华  杨君玲
作者单位:1. 宁夏师范学院医学院形态学教研室,宁夏固原,756000
2. 陕西师范大学有机分析及新药开发实验室,西安,710062
基金项目:宁夏师范学院一般科研资助项目
摘    要:细菌标本片在《病原生物学》教学过程中占有举足轻重的地位,需要大量的永久标本片。其制作要求背景洁净、分布均匀、染色鲜艳、物象清晰、形态典型、结构完整、大小准确、规律排列、省时省力、耗材量少、成本低廉。制作永久保存的细菌标本片是十分复杂繁琐的过程,而且对技术的精、准要求非常严格。文中用动态载片培养法能批量制作永久标本片且满足以上要求,在标本片的永久保存和提高教学质量方面取得了良好的效果。

关 键 词:细菌  永久标本片  动态培养  批量制作

Research and Development of Bacteria Film Specimens for -Dynamic Batch-culture Slides
YANG Dong-hua,YANG Jun-ling. Research and Development of Bacteria Film Specimens for -Dynamic Batch-culture Slides[J]. Experiment Science & Technology, 2012, 10(4): 26-29
Authors:YANG Dong-hua  YANG Jun-ling
Affiliation:1.Laboratory of Morphology,Medicine Department of Ningxia Teachers College,Guyuan 756000,China; 2.Laboratory of Organic Analysis and Drug Discovery,Shanxi Normal University,Xi’ an 710062,China)
Abstract:The specimen slide of bacterium occupies a crucial position in the teaching process of pathogenic biology,which requires a great quantity of the permanent specimen slide.The process of making specimen slide with clean background,uniform distribution,bright dyed,clear physical image,typical shape,complete structure,accurate size,inerratic arrangement,effort and efficiency,less consumption,low cost,permanent preservation are rigmarole,the requirement is very strict in technology.The above requirements are satisfied by dynamic slide culturation which produce permanent specimen films massively in this paper.Furthermore,it has achieved good effects in the preservation of specimen and the improvement of teaching quality.
Keywords:bacterium  permanent specimen films  dynamic cultivation  mass production
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