| 不同产地佛手水溶性多糖的分离纯化及初步分析 |
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| 引用本文： | 曹诣斌,朱海玲,王晓艳.不同产地佛手水溶性多糖的分离纯化及初步分析[J].浙江师范大学学报(自然科学版),2008,31(2):190-194. |
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| 作者姓名： | 曹诣斌 朱海玲 王晓艳 |
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| 作者单位： | 浙江师范大学,化学与生命科学学院,浙江,金华,321004 |
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| 摘 要： | 醇提后的佛手经热水提取并除去蛋白质，乙醇沉淀得到粗多糖．用DEAE葡聚糖A-50离子交换色谱分离得到不同的多糖：金佛手和建佛手各分离得到3种多糖，川佛手分离得到4种多糖．将佛手多糖经酸水解后用硅胶G薄层色谱分析，金佛手多糖和建佛手多糖各检测到3种单糖成分：甘露糖、葡萄糖和半乳糖，川佛手多糖检测到5种单糖成分：鼠李糖、木糖、甘露糖、葡萄糖和半乳糖．红外光谱分析表明，3种产地佛手多糖的吸收光谱均具有典型的吡喃糖特征吸收峰；同时，金佛手多糖还具有较强的呋喃环特征吸收峰．
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| 关 键 词： | 佛手 水溶性多糖 提取 纯化 单糖 |
| 文章编号： | 1001-5051(2008)02-0190-05 |
| 修稿时间： | 2007年10月29 |
Preliminary analysis on purification of the water-soluble polysaccharides in bergamots from different areas |
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| CAO Yibin,ZHU Hailing,WANG Xiaoyan.Preliminary analysis on purification of the water-soluble polysaccharides in bergamots from different areas[J].Journal of Zhejiang Normal University Natural Sciences,2008,31(2):190-194. |
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| Authors: | CAO Yibin ZHU Hailing WANG Xiaoyan |
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| Institution: | (College of Chemistry and Life Science, Zhefiang Normal University, Jinhua Zhejiang 321004, China) |
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| Abstract: | Polysaccharides in bergamots from different areas were extracted and precipitated by ethanol, and separated on DEAE sephadex A-50 ion exchange column. There were three purified polysaccharides in both Jin Bergamot and Jian Bergamot, and four purified polysaccharides in Chuan Bergamot. The components of monosaccharide in Jin Bergamot and Jian Bergamot were mannose, glucose and galactose, and the components of monosaccharide in Jin Bergamot were rhamnose, xylose, mannose, glucose and galactose. The results of infrared spectroscopy assay indicated that the monosaccharides in the polysaccharides of Jin Bergamot, Chuan Bergamot and Jian Bergamot contain pyranoside, and the polysaccharides of Jin Bergamot also contained furanoside. |
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| Keywords: | bergamot polysaccharide isolation purification monosaccharide |
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