MAPK regulates cell cycle progression in pig oocytes and fertilized eggs |
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Authors: | Manyu?Li Hengyu?Fan Chao?Tong Dayuan?Chen Guoliang?Xia Email author" target="_blank">Qingyuan?SunEmail author |
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Institution: | (1) State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China;(2) College of Biological Sciences, China Agricultural University, 100094 Beijing, China;(3) College of Veterinary Medicine, China Agricultural University, 100094 Beijing, China |
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Abstract: | Oocytes collected from prepubertal gilt ovaries were matured in vitro (IVM), and fertilized in vitro (IVF) or electrically activated. Phosphorylation of mitogenactivated protein kinase (MAPK) was detected with SDS-PAGE and
Western blotting, and translocation of ERK2 was observed with immunofluorescent cytochemistry. We found that the quantity of MAPK kept unchanged during oocyte maturation.
There was no phosphorylated MAPK in porcine oocytes at the germinal vesicle (GV) stage; a little MAPK was phosphorylated at
20 h of IVM; a high level phosphorylation was detected at 30 h, while MAPK phosphorylation decreased at 36 h; and then MAPK
phosphorylation increased again to the peak level from 40 to 60 h. ERK2 translocated from the peripheral cytoplasm to inner
cytoplasm and nuclear area during oocyte maturation. There was nearly no phosphorylated MAPK at 18 and 20 h of electrically
activated oocytes, but phosphorylation increased at 22 h. There was no phosphorylated MAPK at 12 h of IVF, while phosphorylation
resumed at 16 h. These results suggest that MAPK may play an important regulatory role in MI-MII transition, pronucleus formation
and the initiation of the first mitosis in pig eggs. |
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Keywords: | porcine oocyte MAPK IVM IVF |
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