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| 试剂盒法提取蓖麻基因组DNA的条件优化 | |
| 引用本文： | 黄凤兰,郭志强,孟凡娟,张继星,孙鹏,李静,刘爽.试剂盒法提取蓖麻基因组DNA的条件优化[J].内蒙古民族大学学报(自然科学版),2010,25(1):29-33. |
| 作者姓名： | 黄凤兰 郭志强 孟凡娟 张继星 孙鹏 李静 刘爽 |
| 作者单位： | 1. 内蒙古民族大学,生命科学学院,内蒙古,通辽,028000;内蒙古自治区高校蓖麻产业研究工程中心,内蒙古,通辽,028042 2. 内蒙古民族大学,生命科学学院,内蒙古,通辽,028000 3. 东北林业大学,生命科学学院,黑龙江,哈尔滨,150040 |
| 基金项目： | 国家自然科学基金项目(30760123);;内蒙古自治区高校蓖麻产业研究工程中心资助项目 |
| 摘 要： | 利用DNA提取试剂盒提取蓖麻叶片DNA,对其过程进行改良优化,从而获得一种快速、简便的提取蓖麻高质量基因组DNA的方法.在原试剂盒的操作基础上,对乙醇是否冰预冷、消化时间、洗脱液用量、材料用量等条件进行了优化,并对所提取的基因组DNA的浓度、纯度进行了检测.优化后的方法提取到了较高质量的蓖麻基因组DNA. |
| 关 键 词： | 蓖麻 基因组DNA 试剂盒 |
Optimizing Condition of Extracting Genomic DNA on Ricinus Communis L.by Kit |
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| HUAGN Feng-lan,GUO Zhi-qiang,MENG Fan-juan,ZHANG Ji-xing,SUN Peng,LI Jing,LIU Shuang.Optimizing Condition of Extracting Genomic DNA on Ricinus Communis L.by Kit[J].Journal of Inner Mongolia University for the Nationalities(Natural Sciences),2010,25(1):29-33. | |
| Authors: | HUAGN Feng-lan GUO Zhi-qiang MENG Fan-juan ZHANG Ji-xing SUN Peng LI Jing LIU Shuang |
| Institution: | 1.College of Life Science/a>;Inner Mongolia University for Nationalities/a>;Tongliao 028000/a>;China/a>; |
| Abstract: | The genomic DNA of leaf on Ricinus communis L was extracted and the process was optimizing.Thereby a fast and convenient method of extracting genomic DNA of leaf on Ricinus communis L was accepted.Being based on origining kit,the conditions including to precooling alcohol by ice,digestion period,eluant dosis and materials dosis were optimized.The density and purity of genomic DNA were detected.Accordingly high-quality genomic DNA of leaf on Ricinus communis L was accepted. |
| Keywords: | Ricinus communis L Genomic DNA Kit |
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