| HPLC法测定人体尿液中有机酸的含量 |
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| 引用本文： | 高素莲,周宁国,王雪梅.HPLC法测定人体尿液中有机酸的含量[J].安徽大学学报(自然科学版),2005,29(4):75-78. |
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| 作者姓名： | 高素莲 周宁国 王雪梅 |
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| 作者单位： | 1. 安徽大学,化学化工学院,安徽,合肥,230039
2. 甘肃长颖投资开发有限公司,甘肃,兰州,730000 |
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| 摘 要： | 以苯酚为内标物,用反相高效液相色谱法(RP-HPLC)测定了人体晨尿中尿酸(UA)、对-氨基苯甲酸(P-ABA)、马尿酸(HA)、高香草酸(HVA)的含量;探讨了尿样预处理技术。该法的检测限为0.23~0.59ng;回收率为97.65%~98.49%;相对标准偏差为1.05%~2.73%。本文建立的分析方法可满足临床胃癌、肺癌等病人的尿液中有机酸的监测与诊断。
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| 关 键 词： | 高效液相色谱法 尿酸 马尿酸 高香草酸 对-氨基苯甲酸 |
| 文章编号： | 1000-2162(2005)04-0075-04 |
Determination of organic acid in human urine by HPLC |
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| GAO Su-lian,ZHOU Ning-guo,WANG Xue-mei.Determination of organic acid in human urine by HPLC[J].Journal of Anhui University(Natural Sciences),2005,29(4):75-78. |
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| Authors: | GAO Su-lian ZHOU Ning-guo WANG Xue-mei |
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| Institution: | GAO Su-lian1,ZHOU Ning-guo2,WANG Xue-mei1 |
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| Abstract: | The content of uric acid,p-Aminobenzonic acid,Hippuric acid,Homovanillic acid in human’s urine were determinated by Reversed-phase High-Performance Liquid Chromatography,using phenol as internal standard.The pre-treatment technique of urine was discussed briefly.The recoveries are from 97.65% to 98.49%,and the relative standard deviations are from 1.05% to 2.73%,and the detection limits are from 0.23 to 0.59 ng.The analytical method is suitable for the routine monitoring and diagnosis of organic acid in cancer's urine. |
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| Keywords: | high-performance liquid chromatography uric acid hippuric acid homovanillic acid p-Aminobenzonic acid |
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