阿特拉津降解细菌的直接检测及分离

摘要：利用改良的 SM固体培养基，对受除草剂污染的土壤中阿特拉津降解菌进行了检测、计数及分离。研究发现，在工业污染土壤中，阿特拉津降解菌群成为微生物群落的主要组成部分，而在农业土壤中仅占次要地位。用多重PCR分析了靶向基因trzN 和 atzABC，表明含trzN-atzBC基因组合的降解菌在山东省工业及农业土壤中占主导地位。因为富集偏差的消除，直接分离方法更利于阿特拉津降解菌多样性分析的研究。

Direct detection and isolation of atrazine-degrading bacteria

We detected, enumerated and isolated atrazine degrading bacteria from herbicide contaminated soil by improved agar SM. We found that atrazine degraders constituted a major component of microbial communities in industrially contaminated soils, but a minor component in agricultural soils. We also analyzed targeting genes trzN and atzABC with multiplex PCR, which indicated that trzN and atzABC combination included degraders were prevalent in Shandong agricultural and industrial soils. Due to elimination of enrichment bias, direct isolation was more favorable for the diversity analysis of atrazine degrading bacteria.