| 高效电转化率条件的探索 |
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| 引用本文： | 张建琼,张雪萍,谢维,单祥年,林陵.高效电转化率条件的探索[J].南京师大学报,2000,23(2):72-75. |
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| 作者姓名： | 张建琼 张雪萍 谢维 单祥年 林陵 |
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| 作者单位： | [1]南京铁道医学院微生物免疫学教研室 [2]南京铁道医学院生物学教研室 |
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| 基金项目： | 江苏省应用基础基金!资助 (BJ970 70 ) |
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| 摘 要： | 对影响ＤＮＡ电转化效率的主要因素进行探索，结果表明：在相同的电场强度、电阻入电容条件下，电中时间的长短对电转化交谊级较大影响，电脉冲时间太长或太短均使转化效率降低；用对数生长早中期的细菌制备感受态细胞，经液氮速冻后储存于－７０℃，能获得高转化效率。
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| 关 键 词： | 大肠杆菌 电转化 转化效率 噬菌体抗体库 DNA |
| 修稿时间： | 1999-09-05 |
Survey the Highest of Transformations-efficiency of the Competent Cell |
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| ZHANG Jian-qiong,ZHANG Xue-ping,XIE Wei,SHANG Xiang-nian,LIN Ling.Survey the Highest of Transformations-efficiency of the Competent Cell[J].Journal of Nanjing Normal University(Natural Science Edition),2000,23(2):72-75. |
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| Authors: | ZHANG Jian-qiong ZHANG Xue-ping XIE Wei SHANG Xiang-nian LIN Ling |
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| Abstract: | Transformation-efficiency of the competent cell is one of the important factors that affects combinatorial library capacity.Some factors about transformation efficiency were compared and analyzed and the condition about the highest transforming efficiency was obtained.The present experiments demonstrate that the optical density at 600 nm of the E.coli XL 1 Blue is not more than 0.5,and electrocompetent XL 1 Blue placed in liquid nitrogen that they freeze instantaneously,and set up the Gene pulser to 2.5 kV,using a 25 uF capacitor and the pulse controller to 200 Ohm.Pulse once (press the button for two seconds after the signal tone) will obtain highly transforming efficiency. |
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| Keywords: | E coli XL 1 Blue electrotransfer transformation efficiency |
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