| 小鼠附睾精子的显微受精 |
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| 引用本文： | 曹佐武,金鹰,钟瑜.小鼠附睾精子的显微受精[J].暨南大学学报,2000,21(4):46-50. |
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| 作者姓名： | 曹佐武 金鹰 钟瑜 |
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| 作者单位： | 1. 暨南大学生殖免疫研究中心,广东,广州,510632
2. 华南师范大学激光生命科学研究所,广东,广州,510631 |
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| 基金项目： | 国务院侨办重点学科科研基金(93-95-29)资助项目 |
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| 摘 要： | 目的：建立显微受精的方法，并探讨小鼠附睾精子在显微注射进入卵母细胞后的受精能力。方法：用显微注射法把小鼠附睾头和附睾尾精子注入卵母细胞的胞质内或卵周隙进行显微受精。结果：把单个附睾尾精子注入卵细胞质中，培养后１４个存活的卵细胞中，有５个卵裂为２－细胞期胚胎；将单个附睾头精子注入卵母细胞中，有２５个存活，其中９个受精发育为２－细胞期胚胎；将附睾尾精子注入卵周隙进行带下受精，３０个存活的卵细胞中有４个
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| 关 键 词： | 显微受精 附睾精子 小鼠 受精率 体外受精 |
| 文章编号： | 1000-9965(2000)04-0046-05 |
| 修稿时间： | 1999-06-11 |
Microfertilization by epididymal sperm of mouse |
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| CAO Zuo-wu,JIN Ying,ZHONG Yu.Microfertilization by epididymal sperm of mouse[J].Journal of Jinan University(Natural Science & Medicine Edition),2000,21(4):46-50. |
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| Authors: | CAO Zuo-wu JIN Ying ZHONG Yu |
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| Institution: | CAO Zuo-wu
;(Center for Reproductive Immunology Research,Jinan University,Guangzhou 510632,China);JIN Ying
;(The Institute of Laser and Life Science,South China Normal University,Guangzhou 510631,China);ZHONG Yu
;(Center for Reproductive Immunology Research,Jinan University,Guangzhou 510632,China) |
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| Abstract: | Aim: To establish the methods of microfertilization and explore the fertilizing potential of mouse sperm obtained from caput epididymidis and cauda epididymidis. Methods: ICSI and SUZI were employed to obtain the assisted fertilization of mouse sperm from caput epididymidis and cauda epididymidis. Results:Single epididymal sperm was injected intracytoplasmically(ICSI) into mouse oocytes. Of 14 survival ooctyes injected with cauda epididymal sperm, 5 oocytes were fertilized and developed to 2-cell stage. Whereas 25 oocytes survied the intracytoplasmic injection of single caput epididymal sperm, 9 of which fertilized and cleaved to 2-cell stage. Subzonal insemination(SUZI) was carried out by injection of 1-3 cauda epididymal sperm into perivitelline space of mouse oocyte. 4 out of 30 SUZI oocytes fertilized. Conclusion: These results demonstrate that we have succeed in the fertilization of mouse oocytes by micro-injection of epididymal sperm, but the fertilization rates remain to be improved. |
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| Keywords: | microfertilization(SUZI/ICSI) epididymal sperm mouse |
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